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Molecular biology

Cloning, primers and plasmid maps in the browser

Check a cloning step before you touch a pipette. Design the primers, build the construct and pick the digest that confirms it.

Start here: Diagnostic restriction digest picker with a schematic gel

How the work runs

  1. Design primers for your insert, with their melting temperatures side by side.

    Hand-off to Plasmid

  2. Cut the vector and insert, and see the ligated construct.

  3. Choose the digest that screens your colonies.

Tools and worked examples

Each opens ready to use, with the method beside the answer.

Reference pages

Reference tables and worked examples, with their data and methods.

Plasmid

Reference tables: Restriction enzymes, Restriction digests of common cloning vectors

Where the work is kept

In Bench, a Study holds the question, the work you did, the results it produced and the materials and sources behind them, ready for the next time you return.

Cloud backup and cross-device sync are not enabled in the current browser preview.

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