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Drybench

Restriction digests of two cloning vectors

One page for each pairing of a vector, pUC19 or pBR322, with an enzyme that cuts it at between 2 and 8 sites, every site being cut both in unmethylated DNA and in DNA with dam and dcm methylation. Each page lists the fragments and cut positions and opens the digest in Drybench Plasmid.

Selection: enzymes of enzyme table version 1 meeting that rule. Enzymes with the same site and the same fragment lengths share a page. Enzymes that cut once are listed on each vector's map page instead, and enzymes with a site whose cut depends on methylation have no digest page.

DigestSitesFragments (bp)
pBR322 digested with AccI22,768, 1,593
pBR322 digested with AclI41,792, 1,297, 899, 373
pBR322 digested with AcuI23,349, 1,012
pBR322 digested with AfeI42,866, 952, 281, 262
pBR322 digested with ApaLI32,617, 1,246, 498
pBR322 digested with BaeGI32,617, 1,246, 498
pBR322 digested with BanII24,347, 14
pBR322 digested with BbsI32,751, 863, 747
pBR322 digested with BceAI31,996, 1,808, 557
pBR322 digested with BciVI22,834, 1,527
pBR322 digested with BfaI51,479, 1,259, 1,035, 335, 253
pBR322 digested with BglI32,317, 1,810, 234
pBR322 digested with BmrI51,540, 1,254, 1,192, 321, 54
pBR322 digested with BpmI41,757, 1,434, 616, 554
pBR322 digested with BpuEI61,709, 1,075, 868, 298, 241, 170
pBR322 digested with BsaHI62,697, 657, 490, 382, 114, 21
pBR322 digested with BseYI22,533, 1,828
pBR322 digested with BsiHKAI81,161, 824, 604, 587, 498, 311, 291, 85
pBR322 digested with BsmAI32,274, 1,311, 776
pBR322 digested with BsmFI43,167, 648, 321, 225
pBR322 digested with BspCNI72,079, 540, 519, 486, 409, 166, 162
pBR322 digested with BspHI42,704, 1,008, 544, 105
pBR322 digested with BsrBI22,560, 1,801
pBR322 digested with BsrDI24,187, 174
pBR322 digested with BsrFI72,163, 1,075, 359, 354, 241, 160, 9
pBR322 digested with BssSI32,670, 1,384, 307
pBR322 digested with BstAPI23,115, 1,246
pBR322 digested with BstNI61,857, 1,058, 929, 383, 121, 13
pBR322 digested with BstYI81,447, 1,292, 768, 728, 86, 17, 12, 11
pBR322 digested with BtgI23,442, 919
pBR322 digested with BtgZI32,892, 1,181, 288
pBR322 digested with BtsI24,341, 20
pBR322 digested with DdeI81,653, 540, 540, 465, 426, 409, 166, 162
pBR322 digested with DraI33,650, 692, 19
pBR322 digested with DrdI23,948, 413
pBR322 digested with EarI22,557, 1,804
pBR322 digested with EciI42,235, 1,152, 828, 146
pBR322 digested with FspAI23,167, 1,194
pBR322 digested with FspI42,132, 1,096, 1,035, 98
pBR322 digested with HincII23,254, 1,107
pBR322 digested with Hpy166II81,136, 979, 630, 498, 488, 457, 128, 45
pBR322 digested with MluCI81,900, 1,068, 565, 306, 255, 193, 60, 14
pBR322 digested with MlyI41,726, 1,647, 502, 486
pBR322 digested with MmeI42,379, 1,711, 184, 87
pBR322 digested with MspA1I61,501, 941, 925, 630, 245, 119
pBR322 digested with NaeI43,479, 368, 354, 160
pBR322 digested with NarI43,569, 657, 114, 21
pBR322 digested with NmeAIII32,367, 1,052, 942
pBR322 digested with NspI42,450, 1,254, 365, 292
pBR322 digested with PflMI24,312, 49
pBR322 digested with PleI41,727, 1,646, 503, 485
pBR322 digested with RsaI32,116, 1,565, 680
pBR322 digested with SfcI42,600, 892, 678, 191
pBR322 digested with SmlI61,709, 1,039, 868, 277, 262, 206
pBR322 digested with TfiI62,765, 504, 419, 298, 221, 154
pBR322 digested with XmnI22,429, 1,932
pUC19 digested with AclI22,313, 373
pUC19 digested with AcuI21,674, 1,012
pUC19 digested with ApaLI31,246, 943, 497
pUC19 digested with AseI31,392, 1,235, 59
pUC19 digested with AvaII22,464, 222
pUC19 digested with BaeGI31,246, 943, 497
pUC19 digested with BanI41,274, 1,097, 173, 142
pUC19 digested with BccI32,275, 287, 124
pUC19 digested with BceAI21,781, 905
pUC19 digested with BciVI21,527, 1,159
pUC19 digested with BfaI41,221, 877, 335, 253
pUC19 digested with BglI21,568, 1,118
pUC19 digested with BmrI21,380, 1,306
pUC19 digested with BpuEI41,279, 868, 298, 241
pUC19 digested with BsaHI32,000, 382, 304
pUC19 digested with BsaWI31,708, 831, 147
pUC19 digested with BsiHKAI51,161, 718, 497, 225, 85
pUC19 digested with BslI61,448, 601, 279, 174, 166, 18
pUC19 digested with BsmAI41,715, 776, 153, 42
pUC19 digested with BsmBI22,644, 42
pUC19 digested with Bsp1286I51,161, 718, 497, 225, 85
pUC19 digested with BspCNI5931, 661, 519, 409, 166
pUC19 digested with BspHI31,573, 1,008, 105
pUC19 digested with BsrBI31,801, 644, 241
pUC19 digested with BsrDI22,512, 174
pUC19 digested with BssSI31,384, 995, 307
pUC19 digested with BstNI52,073, 288, 191, 121, 13
pUC19 digested with BstYI71,030, 768, 762, 86, 17, 12, 11
pUC19 digested with BtsCI51,351, 623, 287, 244, 181
pUC19 digested with BtsI31,507, 1,159, 20
pUC19 digested with DdeI6910, 540, 426, 409, 235, 166
pUC19 digested with DraI31,975, 692, 19
pUC19 digested with DrdI21,869, 817
pUC19 digested with EaeI31,442, 987, 257
pUC19 digested with EarI31,804, 488, 394
pUC19 digested with EciI31,712, 828, 146
pUC19 digested with FauI52,145, 329, 160, 42, 10
pUC19 digested with FokI51,331, 643, 287, 244, 181
pUC19 digested with FspI21,663, 1,023
pUC19 digested with HaeII31,871, 445, 370
pUC19 digested with HgaI4820, 730, 578, 558
pUC19 digested with HinfI61,419, 517, 396, 214, 75, 65
pUC19 digested with HphI71,509, 416, 284, 227, 206, 35, 9
pUC19 digested with Hpy166II5758, 691, 497, 488, 252
pUC19 digested with Hpy99I51,094, 794, 522, 263, 13
pUC19 digested with HpyAV6782, 527, 455, 359, 310, 253
pUC19 digested with HpyCH4III8568, 515, 506, 470, 313, 208, 71, 35
pUC19 digested with HpyCH4IV51,135, 442, 416, 373, 320
pUC19 digested with MluCI7988, 955, 306, 255, 90, 75, 17
pUC19 digested with MlyI41,434, 502, 486, 264
pUC19 digested with MmeI22,502, 184
pUC19 digested with MslI32,168, 359, 159
pUC19 digested with MspA1I6941, 518, 466, 322, 245, 194
pUC19 digested with NspI31,917, 404, 365
pUC19 digested with PleI41,433, 503, 485, 265
pUC19 digested with PvuI21,790, 896
pUC19 digested with PvuII22,364, 322
pUC19 digested with RsaI31,769, 676, 241
pUC19 digested with Sau96I61,455, 616, 297, 222, 79, 17
pUC19 digested with SfaNI81,052, 665, 359, 249, 191, 94, 40, 36
pUC19 digested with SfcI41,181, 678, 636, 191
pUC19 digested with SmlI41,279, 868, 277, 262
pUC19 digested with TaqI41,444, 736, 476, 30
pUC19 digested with TfiI22,546, 140
pUC19 digested with Tsp45I41,588, 576, 311, 211

For research and education. This page is not medical, legal or investment advice.