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Assay analysis

IC50 fitting, Z prime and standard curves online

Fit, check and report plate data with the model and interval stated, so a reader can repeat it.

Start here: IC50 and EC50 calculator with confidence intervals

How the work runs

  1. Plan the dilution series and its plate.

    Hand-off to Assay

  2. Fit one curve per compound.

  3. Check that the controls separate signal from background.

  4. Report each IC50 or EC50 with its model and interval.

Tools and worked examples

Each opens ready to use, with the method beside the answer.

Where the work is kept

In Bench, a Study holds the question, the work you did, the results it produced and the materials and sources behind them, ready for the next time you return.

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