Restriction enzymes
One page for each enzyme in Drybench Plasmid's enzyme table that has at least one recognition site in pUC19 or pBR322. Each page gives the enzyme's site and cut, the ends it leaves, its recorded dam and dcm sensitivity and where it cuts each vector, and opens the map with the enzyme shown.
Selection: every enzyme of enzyme table version 1 with a site on pUC19 or pBR322, in alphabetical order. Enzymes without a site on either vector have no page.
| Enzyme | Recognition site | Ends |
|---|---|---|
| AatII | GACGTC | a 4-base 3′ overhang (ACGT) |
| Acc65I | GGTACC | a 4-base 5′ overhang (GTAC) |
| AccI | GTMKAC | a 2-base 5′ overhang (MK) |
| AciI | CCGC | a 2-base 5′ overhang (CG) |
| AclI | AACGTT | a 2-base 5′ overhang (CG) |
| AcuI | CTGAAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| AfeI | AGCGCT | blunt ends |
| AflIII | ACRYGT | a 4-base 5′ overhang (CRYG) |
| AhdI | GACNNNNNGTC | a 1-base 3′ overhang (N) |
| AluI | AGCT | blunt ends |
| AlwI | GGATC | a 1-base 5′ overhang whose bases are not fixed by the recognition site |
| AlwNI | CAGNNNCTG | a 3-base 3′ overhang (NNN) |
| ApaLI | GTGCAC | a 4-base 5′ overhang (TGCA) |
| ApeKI | GCWGC | a 3-base 5′ overhang (CWG) |
| ApoI | RAATTY | a 4-base 5′ overhang (AATT) |
| AseI | ATTAAT | a 2-base 5′ overhang (TA) |
| AvaI | CYCGRG | a 4-base 5′ overhang (YCGR) |
| AvaII | GGWCC | a 3-base 5′ overhang (GWC) |
| BaeGI | GKGCMC | a 4-base 3′ overhang (KGCM) |
| BamHI | GGATCC | a 4-base 5′ overhang (GATC) |
| BanI | GGYRCC | a 4-base 5′ overhang (GYRC) |
| BanII | GRGCYC | a 4-base 3′ overhang (RGCY) |
| BbsI | GAAGAC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BbvI | GCAGC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BccI | CCATC | a 1-base 5′ overhang whose bases are not fixed by the recognition site |
| BceAI | ACGGC | a 2-base 5′ overhang whose bases are not fixed by the recognition site |
| BciVI | GTATCC | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| BfaI | CTAG | a 2-base 5′ overhang (TA) |
| BglI | GCCNNNNNGGC | a 3-base 3′ overhang (NNN) |
| BmrI | ACTGGG | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| BmtI | GCTAGC | a 4-base 3′ overhang (CTAG) |
| BpmI | CTGGAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| Bpu10I | CCTNAGC | a 3-base 5′ overhang (TNA) |
| BpuEI | CTTGAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BsaAI | YACGTR | blunt ends |
| BsaBI | GATNNNNATC | blunt ends |
| BsaHI | GRCGYC | a 2-base 5′ overhang (CG) |
| BsaI | GGTCTC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BsaJI | CCNNGG | a 4-base 5′ overhang (CNNG) |
| BsaWI | WCCGGW | a 4-base 5′ overhang (CCGG) |
| BseYI | CCCAGC | a 4-base 5′ overhang (CCAG) |
| BsgI | GTGCAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BsiEI | CGRYCG | a 2-base 3′ overhang (RY) |
| BsiHKAI | GWGCWC | a 4-base 3′ overhang (WGCW) |
| BslI | CCNNNNNNNGG | a 3-base 3′ overhang (NNN) |
| BsmAI | GTCTC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BsmBI | CGTCTC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BsmFI | GGGAC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BsmI | GAATGC | a 2-base 3′ overhang (CN); bases shown as N are not fixed by the recognition site |
| Bsp1286I | GDGCHC | a 4-base 3′ overhang (DGCH) |
| BspCNI | CTCAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BspEI | TCCGGA | a 4-base 5′ overhang (CCGG) |
| BspHI | TCATGA | a 4-base 5′ overhang (CATG) |
| BspMI | ACCTGC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BsrBI | CCGCTC | blunt ends |
| BsrDI | GCAATG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BsrFI | RCCGGY | a 4-base 5′ overhang (CCGG) |
| BsrI | ACTGG | a 2-base 3′ overhang (GN); bases shown as N are not fixed by the recognition site |
| BssSI | CACGAG | a 4-base 5′ overhang (ACGA) |
| BstAPI | GCANNNNNTGC | a 3-base 3′ overhang (NNN) |
| BstNI | CCWGG | a 1-base 5′ overhang (W) |
| BstUI | CGCG | blunt ends |
| BstYI | RGATCY | a 4-base 5′ overhang (GATC) |
| BstZ17I | GTATAC | blunt ends |
| BtgI | CCRYGG | a 4-base 5′ overhang (CRYG) |
| BtgZI | GCGATG | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| BtsCI | GGATG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BtsI | GCAGTG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| BtsIMutI | CAGTG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| Cac8I | GCNNGC | blunt ends |
| ClaI | ATCGAT | a 2-base 5′ overhang (CG) |
| CviAII | CATG | a 2-base 5′ overhang (AT) |
| CviKI-1 | RGCY | blunt ends |
| CviQI | GTAC | a 2-base 5′ overhang (TA) |
| DdeI | CTNAG | a 3-base 5′ overhang (TNA) |
| DpnI | GATC | blunt ends |
| DpnII | GATC | a 4-base 5′ overhang (GATC) |
| DraI | TTTAAA | blunt ends |
| DrdI | GACNNNNNNGTC | a 2-base 3′ overhang (NN) |
| EaeI | YGGCCR | a 4-base 5′ overhang (GGCC) |
| EagI | CGGCCG | a 4-base 5′ overhang (GGCC) |
| EarI | CTCTTC | a 3-base 5′ overhang whose bases are not fixed by the recognition site |
| EciI | GGCGGA | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| Eco53kI | GAGCTC | blunt ends |
| EcoNI | CCTNNNNNAGG | a 1-base 5′ overhang (N) |
| EcoO109I | RGGNCCY | a 3-base 5′ overhang (GNC) |
| EcoRI | GAATTC | a 4-base 5′ overhang (AATT) |
| EcoRII | CCWGG | a 5-base 5′ overhang (CCWGG) |
| EcoRV | GATATC | blunt ends |
| FatI | CATG | a 4-base 5′ overhang (CATG) |
| FauI | CCCGC | a 2-base 5′ overhang whose bases are not fixed by the recognition site |
| Fnu4HI | GCNGC | a 1-base 5′ overhang (N) |
| FokI | GGATG | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| FspAI | RTGCGCAY | blunt ends |
| FspI | TGCGCA | blunt ends |
| HaeII | RGCGCY | a 4-base 3′ overhang (GCGC) |
| HaeIII | GGCC | blunt ends |
| HgaI | GACGC | a 5-base 5′ overhang whose bases are not fixed by the recognition site |
| HhaI | GCGC | a 2-base 3′ overhang (CG) |
| HincII | GTYRAC | blunt ends |
| HindIII | AAGCTT | a 4-base 5′ overhang (AGCT) |
| HinfI | GANTC | a 3-base 5′ overhang (ANT) |
| HinP1I | GCGC | a 2-base 5′ overhang (CG) |
| HpaII | CCGG | a 2-base 5′ overhang (CG) |
| HphI | GGTGA | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| Hpy166II | GTNNAC | blunt ends |
| Hpy188I | TCNGA | a 1-base 3′ overhang (N) |
| Hpy188III | TCNNGA | a 2-base 5′ overhang (NN) |
| Hpy99I | CGWCG | a 5-base 3′ overhang (CGWCG) |
| HpyAV | CCTTC | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| HpyCH4III | ACNGT | a 1-base 3′ overhang (N) |
| HpyCH4IV | ACGT | a 2-base 5′ overhang (CG) |
| HpyCH4V | TGCA | blunt ends |
| KasI | GGCGCC | a 4-base 5′ overhang (GCGC) |
| KpnI | GGTACC | a 4-base 3′ overhang (GTAC) |
| MboI | GATC | a 4-base 5′ overhang (GATC) |
| MboII | GAAGA | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| MluCI | AATT | a 4-base 5′ overhang (AATT) |
| MlyI | GAGTC | blunt ends |
| MmeI | TCCRAC | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| MnlI | CCTC | a 1-base 3′ overhang whose bases are not fixed by the recognition site |
| MscI | TGGCCA | blunt ends |
| MseI | TTAA | a 2-base 5′ overhang (TA) |
| MslI | CAYNNNNRTG | blunt ends |
| MspA1I | CMGCKG | blunt ends |
| MspI | CCGG | a 2-base 5′ overhang (CG) |
| MwoI | GCNNNNNNNGC | a 3-base 3′ overhang (NNN) |
| NaeI | GCCGGC | blunt ends |
| NarI | GGCGCC | a 2-base 5′ overhang (CG) |
| NdeI | CATATG | a 2-base 5′ overhang (TA) |
| NgoMIV | GCCGGC | a 4-base 5′ overhang (CCGG) |
| NheI | GCTAGC | a 4-base 5′ overhang (CTAG) |
| NlaIII | CATG | a 4-base 3′ overhang (CATG) |
| NlaIV | GGNNCC | blunt ends |
| NmeAIII | GCCGAG | a 2-base 3′ overhang whose bases are not fixed by the recognition site |
| NruI | TCGCGA | blunt ends |
| NspI | RCATGY | a 4-base 3′ overhang (CATG) |
| PciI | ACATGT | a 4-base 5′ overhang (CATG) |
| PflMI | CCANNNNNTGG | a 3-base 3′ overhang (NNN) |
| PleI | GAGTC | a 1-base 5′ overhang whose bases are not fixed by the recognition site |
| PluTI | GGCGCC | a 4-base 3′ overhang (GCGC) |
| PpuMI | RGGWCCY | a 3-base 5′ overhang (GWC) |
| PspGI | CCWGG | a 5-base 5′ overhang (CCWGG) |
| PstI | CTGCAG | a 4-base 3′ overhang (TGCA) |
| PvuI | CGATCG | a 2-base 3′ overhang (AT) |
| PvuII | CAGCTG | blunt ends |
| RsaI | GTAC | blunt ends |
| SacI | GAGCTC | a 4-base 3′ overhang (AGCT) |
| SalI | GTCGAC | a 4-base 5′ overhang (TCGA) |
| SapI | GCTCTTC | a 3-base 5′ overhang whose bases are not fixed by the recognition site |
| Sau3AI | GATC | a 4-base 5′ overhang (GATC) |
| Sau96I | GGNCC | a 3-base 5′ overhang (GNC) |
| SbfI | CCTGCAGG | a 4-base 3′ overhang (TGCA) |
| ScaI | AGTACT | blunt ends |
| ScrFI | CCNGG | a 1-base 5′ overhang (N) |
| SfaNI | GCATC | a 4-base 5′ overhang whose bases are not fixed by the recognition site |
| SfcI | CTRYAG | a 4-base 5′ overhang (TRYA) |
| SfoI | GGCGCC | blunt ends |
| SgrAI | CRCCGGYG | a 4-base 5′ overhang (CCGG) |
| SmaI | CCCGGG | blunt ends |
| SmlI | CTYRAG | a 4-base 5′ overhang (TYRA) |
| SphI | GCATGC | a 4-base 3′ overhang (CATG) |
| SspI | AATATT | blunt ends |
| StyD4I | CCNGG | a 5-base 5′ overhang (CCNGG) |
| StyI | CCWWGG | a 4-base 5′ overhang (CWWG) |
| TaqI | TCGA | a 2-base 5′ overhang (CG) |
| TfiI | GAWTC | a 3-base 5′ overhang (AWT) |
| Tsp45I | GTSAC | a 5-base 5′ overhang (GTSAC) |
| TspRI | CASTG | a 9-base 3′ overhang (NNCASTGNN); bases shown as N are not fixed by the recognition site |
| Tth111I | GACNNNGTC | a 1-base 5′ overhang (N) |
| XbaI | TCTAGA | a 4-base 5′ overhang (CTAG) |
| XmaI | CCCGGG | a 4-base 5′ overhang (CCGG) |
| XmnI | GAANNNNTTC | blunt ends |
| ZraI | GACGTC | blunt ends |
For research and education. This page is not medical, legal or investment advice.