NlaIII restriction site and cut
NlaIII recognises CATG and cuts N_CATG^N, leaving a 4-base 3′ overhang (CATG).
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it.
At a glance
| Recognition site | CATG |
|---|---|
| Site length | 4 bases |
| Cut | N_CATG^N |
| Ends | a 4-base 3′ overhang (CATG) |
| REBASE entry | NlaIII on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 11 | 11 | 41, 445, 464, 810, 1,530, 2,021, 2,031, 2,109, 2,145, 2,538, 2,643 |
| pBR322 (J01749.1, 4,361 bp) | 26 | 26 | 8, 355, 493, 566, 712, 751, 937, 1,054, 1,182, 1,197, 1,254, 1,461, 1,595, 1,820, 1,884, 1,947, 2,112, 2,217, 2,477, 3,197, 3,688, 3,698, 3,776, 3,812, 4,205, 4,310 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.