FatI restriction site and cut
FatI recognises CATG and cuts N^CATG_N, leaving a 4-base 5′ overhang (CATG).
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it.
At a glance
| Recognition site | CATG |
|---|---|
| Site length | 4 bases |
| Cut | N^CATG_N |
| Ends | a 4-base 5′ overhang (CATG) |
| REBASE entry | FatI on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 11 | 11 | 37, 441, 460, 806, 1,526, 2,017, 2,027, 2,105, 2,141, 2,534, 2,639 |
| pBR322 (J01749.1, 4,361 bp) | 26 | 26 | 4, 351, 489, 562, 708, 747, 933, 1,050, 1,178, 1,193, 1,250, 1,457, 1,591, 1,816, 1,880, 1,943, 2,108, 2,213, 2,473, 3,193, 3,684, 3,694, 3,772, 3,808, 4,201, 4,306 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.