CviAII restriction site and cut
CviAII recognises CATG and cuts C^AT_G, leaving a 2-base 5′ overhang (AT).
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it.
At a glance
| Recognition site | CATG |
|---|---|
| Site length | 4 bases |
| Cut | C^AT_G |
| Ends | a 2-base 5′ overhang (AT) |
| REBASE entry | CviAII on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 11 | 11 | 38, 442, 461, 807, 1,527, 2,018, 2,028, 2,106, 2,142, 2,535, 2,640 |
| pBR322 (J01749.1, 4,361 bp) | 26 | 26 | 5, 352, 490, 563, 709, 748, 934, 1,051, 1,179, 1,194, 1,251, 1,458, 1,592, 1,817, 1,881, 1,944, 2,109, 2,214, 2,474, 3,194, 3,685, 3,695, 3,773, 3,809, 4,202, 4,307 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.