CviKI-1 restriction site and cut
CviKI-1 recognises RGCY and cuts RG^_CY, leaving blunt ends.
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it. The site uses IUPAC codes: R is A or G; Y is C or T.
At a glance
| Recognition site | RGCY |
|---|---|
| Site length | 4 bases |
| Cut | RG^_CY |
| Ends | blunt ends |
| REBASE entry | CviKI-1 on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 45 | 43 | 44, 63, 96, 136, 140, 254, 288, 308, 390, 404, 449, 471, 523, 544, 566, 630, 647, 729, 748, 821, 832, 850, 876, 974, 1,064, 1,110, 1,115, 1,140, 1,219, 1,284, 1,295, 1,338, 1,367, 1,730, 1,742, 1,783, 1,809, 1,813, 1,822, 1,888, 1,978, 1,988, 2,051, 2,089, 2,676 |
| pBR322 (J01749.1, 4,361 bp) | 73 | 71 | 16, 31, 150, 174, 297, 332, 401, 473, 487, 525, 533, 597, 615, 671, 687, 831, 875, 920, 941, 954, 984, 992, 1,049, 1,090, 1,109, 1,119, 1,257, 1,262, 1,283, 1,327, 1,409, 1,446, 1,488, 1,493, 1,692, 1,948, 1,964, 1,998, 2,055, 2,066, 2,115, 2,134, 2,167, 2,212, 2,252, 2,396, 2,415, 2,488, 2,499, 2,517, 2,543, 2,641, 2,731, 2,777, 2,782, 2,807, 2,886, 2,951, 2,962, 3,005, 3,034, 3,397, 3,409, 3,450, 3,476, 3,480, 3,489, 3,555, 3,645, 3,655, 3,718, 3,756, 4,343 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.