AciI restriction site and cut
AciI recognises CCGC and cuts C^CG_C, leaving a 2-base 5′ overhang (CG).
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it.
At a glance
| Recognition site | CCGC |
|---|---|
| Site length | 4 bases |
| Cut | C^CG_C |
| Ends | a 2-base 5′ overhang (CG) |
| REBASE entry | AciI on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 34 | 34 | 75, 114, 124, 150, 188, 201, 227, 284, 496, 599, 655, 665, 689, 732, 739, 760, 851, 879, 1,006, 1,025, 1,146, 1,256, 1,391, 1,400, 1,762, 1,853, 2,044, 2,090, 2,211, 2,255, 2,332, 2,441, 2,540, 2,587 |
| pBR322 (J01749.1, 4,361 bp) | 67 | 67 | 39, 181, 289, 298, 301, 425, 578, 581, 584, 703, 723, 802, 842, 891, 938, 1,023, 1,038, 1,106, 1,139, 1,164, 1,209, 1,233, 1,245, 1,287, 1,341, 1,394, 1,410, 1,416, 1,497, 1,635, 1,764, 1,767, 1,778, 1,934, 1,950, 2,005, 2,061, 2,146, 2,185, 2,195, 2,237, 2,262, 2,300, 2,313, 2,339, 2,356, 2,399, 2,406, 2,427, 2,518, 2,546, 2,673, 2,692, 2,813, 2,923, 3,058, 3,067, 3,429, 3,520, 3,711, 3,757, 3,878, 3,922, 3,999, 4,108, 4,207, 4,254 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.