MwoI restriction site and cut
MwoI recognises GCNNNNNNNGC and cuts GCNN_NNN^NNGC, leaving a 3-base 3′ overhang (NNN).
In this notation, read on the top strand from five prime to three prime, ^ marks where the top strand is cut and _ where the bottom strand is cut. N stands for any base; Ns before or after the site are flanking bases, not part of it. The site uses IUPAC codes: N is any base.
At a glance
| Recognition site | GCNNNNNNNGC |
|---|---|
| Site length | 11 bases |
| Cut | GCNN_NNN^NNGC |
| Ends | a 3-base 3′ overhang (NNN) |
| REBASE entry | MwoI on REBASE |
Sites in pUC19 and pBR322
| Vector | Sites | Cut under dam and dcm methylation | Cuts after base (top strand) |
|---|---|---|---|
| pUC19 (L09137.2, 2,686 bp) | 13 | 11 | 185, 235, 242, 251, 281, 451, 550, 594, 678, 745, 859, 1,431, 1,819 |
| pBR322 (J01749.1, 4,361 bp) | 34 | 32 | 166, 232, 239, 297, 319, 353, 411, 422, 443, 634, 775, 801, 926, 935, 944, 1,042, 1,046, 1,051, 1,060, 1,160, 1,169, 1,418, 1,427, 1,452, 1,494, 1,559, 1,582, 2,297, 2,347, 2,412, 2,526, 3,098, 3,486, 3,606 |
Counts are for the GenBank record named, as a circle, in that record's numbering. The third column counts the sites cut in DNA with dam and dcm methylation (the Drybench Plasmid default); any other site is blocked, or its outcome is not known. The positions list every recognition site, cut or not. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.