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Drybench Sequence.

Six-frame translation

All six reading frames of a DNA sequence with the stops marked, so the coding frame stands out.

Sequence

Paste once, read every answer

Answer

Six frames

All six frames with the stops marked, so a coding frame stands out.

Paste a sequence, or open an example, and every operation answers.

Open in the workbenchThe workbench carries this paste and offers every operation on it.

Worked example

  • The insulin coding sequence: frame +1 has 1 stop at its end and an open stretch of 110 residues; frames +2 and +3 have 2 and 1 stops, so the coding frame stands out.
  • The three frames of the other strand have 2, 5 and 0 stops.

Method

Frames +1, +2, +3 on the sequence and −1, −2, −3 on its reverse complement

  • Translation with NCBI's genetic code tables. NCBI. The Genetic Codes, gc.prt version 4.6 (last modified 10 June 2020), fetched 2026-10-06. Public domain.Checked against Biopython 1.88 exactly on 108 frame translations of three sequences in genetic codes 1, 2 and 11, full length and to the first stop, and on 5 annotated coding sequences (6 October 2026).

Constants

  • ncbi-genetic-codes-4.6: NCBI genetic code tables, gc.prt version 4.6 (public domain)

Limits

  • For research and education only, and not medical advice: nothing here is an assessment of any person's variant, sample or molecule, and a difference reported from a reference is a difference, not an interpretation.
  • A link keeps the inputs after the # of the address; it is not sent to a server, but it does stay in browser history and in any chat the link is pasted into.
  • Sequence operations run locally. Optional UniProt lookup sends the submitted query and selected accession to rest.uniprot.org, never the separate pasted sequence. The Verify at ProtParam and BLAST at NCBI links upload what you send, and say so; an optional Bench backup uploads the Study you choose.

Verification