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Drybench Sequence.
lacZα: ORFs and six frames (example lacz-alpha@1)
A versioned teaching example: the first 1,500 bases of the E. coli lac operon (J01636.1) with its open reading frames and six-frame translation.
Sequence
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Answer
Open reading frames
ATG to stop by default, 75 nt or longer, nested ORFs hidden; every setting is on the card.
nt
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Worked example
- The first 1,500 bases of the E. coli lac operon (GenBank J01636.1): lacI and the start of lacZ, for the ORF finder and the six frames.
- With the bacterial code (table 11), ATG to stop and 75 nt or longer: 5 ORFs, 6 nested and hidden, 1 running off the end; the six frames show the same stops.
- Source: GenBank J01636.1 (NCBI, public domain).
This page is the example lacz-alpha@1: its method versions are pinned, so it gives the same numbers next year. seq 1.1.0, 2026-10-06.
Method
From a permitted start codon to the next in-frame stop; coding length ≥ the minimum; an ORF inside another is nested
- Open reading frames. NCBI ORFfinder conventions as the CSO set them on 6 October 2026: ATG (or the table's initiation codons) to the table's stop, 75 nt counted to the codon before the stop, nested ORFs hidden, ORFs without a stop listed apart.Checked field by field against an independent scan cross-checked with Biopython 1.88 and NCBI ORFfinder 0.4.3 on 18 cases (6 October 2026).
- Translation with NCBI's genetic code tables. NCBI. The Genetic Codes, gc.prt version 4.6 (last modified 10 June 2020), fetched 2026-10-06. Public domain.Checked against Biopython 1.88 exactly on 108 frame translations of three sequences in genetic codes 1, 2 and 11, full length and to the first stop, and on 5 annotated coding sequences (6 October 2026).
Constants
- ncbi-genetic-codes-4.6: NCBI genetic code tables, gc.prt version 4.6 (public domain)
Limits
- For research and education only, and not medical advice: nothing here is an assessment of any person's variant, sample or molecule, and a difference reported from a reference is a difference, not an interpretation.
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