Drybench Sequence product page
Drybench Sequence.
GC content of a DNA sequence
GC percent with the ambiguity rule stated: S counts, W counts in the total, the other codes are left out.
Sequence
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Answer
GC percent
G and C over all unambiguous bases; S counts as G or C, W counts as A or T, and the other ambiguity codes are left out.
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Worked example
- The insulin coding sequence: 215 of 333 bases are G or C, 64.6 percent.
- S counts as G or C and W as A or T; the other IUPAC codes are left out of both counts, and the card says how many.
Method
GC = (G + C + S) / (A + C + G + T + U + S + W)
Limits
- For research and education only, and not medical advice: nothing here is an assessment of any person's variant, sample or molecule, and a difference reported from a reference is a difference, not an interpretation.
- Tags and protease sites are found by their published motifs and a cut is proposed at the site nearest an end; confirm it, since a motif inside the protein is not a cleavage site.