Drybench Plasmid.
Plasmid ng to pmol and copies calculator
Picomoles, copies per µL and ligation insert mass from the plasmid's own double-stranded weight, beside the 650 Da per bp rule.
From the plasmid's own double-stranded weight, beside the 650 Da per bp rule. For your own plasmid, open it in the map: the Numbers view uses its sequence.
Numbers for this plasmid
From this plasmid's own sequence: its double-stranded weight, and the amounts that follow from it.
Molecular weight
1.65969 × 10⁶ g/mol, double-stranded, circular (no end groups)
The 650 Da per bp rule gives 1.74590 × 10⁶ g/mol; it is an approximation.
ng to pmol
0.06025 pmol
The 650 Da per bp rule gives 0.05728 pmol.
Copies per µL (qPCR standards)
3.628 × 10⁹ copies per µL
ng for a number of copies
0.002756 ng
Ligation insert mass
55.85 ng of insert
The vector length is this plasmid's.
pmol = ng × 1000 / MW; copies = ng × 10⁻⁹ × N_A / MW; insert ng = vector ng × (insert bp / vector bp) × ratio.
How it is computed
- ng, pmol, copies and ligation amounts. pmol = ng × 1000 / MW; copies = ng × 10⁻⁹ × N_A / MW, with N_A = 6.02214076 × 10²³ per mole (exact, SI 2019); insert ng = vector ng × insert bp / vector bp × ratio. MW of the double-stranded plasmid from its own sequence (both strands, no end groups when circular) with the CIAAW 2021 atomic weights.Checked against exact rational arithmetic on 11 cases (pmol, copies per µL, ng for copies, ligation, the 650 Da per bp rule and the weights of pUC19 and pBR322), to full double precision (2026-10-06).
- Nucleic acid molecular weight from the sequence. Residue formulas from the nucleoside 5′-monophosphates (PubChem, read 6 October 2026) less water, summed with the IUPAC CIAAW 2021 atomic weights; 5′-OH and 3′-OH ends as a neutral free acid.Checked against exact rational arithmetic on 11 cases (pmol, copies per µL, ng for copies, ligation, the 650 Da per bp rule and the weights of pUC19 and pBR322), to full double precision (2026-10-06).
Limits
- For research and education only, and not medical advice: plasmid maps and sequence assembly designs. A designed product is not evidence that the reaction occurred; nothing here predicts expression or how well a reaction will work.