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pEGFP-N1 digested with BspMI
BspMI recognises ACCTGC and cuts circular pEGFP-N1 (GenBank U55762.1, 4,733 bp) at 3 sites, so a complete digest gives 3 fragments.
Fragments
Fragment
Length (bp)
From base
To base
1
381
2,645
3,025
2
450
3,026
3,475
3
3,902
3,476
2,644
The fragments add up to 4,733 bp, the length of the record.
Cut sites
Site
Recognition site: first base on the top strand
Site reads on
Cuts after base (top strand)
1
2,653
bottom strand
2,644
2
3,016
top strand
3,025
3
3,466
top strand
3,475
Positions are in the numbering of GenBank U55762.1. A cut after base n leaves base n at the end of one fragment and the next base at the start of the next. A fragment that runs past the record's last base continues from its first. A vendor's map, or your own stock, may differ from this record.
The same question is open below in Drybench Plasmid. Change the enzymes, the host's methylation or the vector there; nothing you do here is saved unless you save it.
Reference dataset active
pEGFP-N1 (U55762.1) digested with BspMI
Opening what was sent…
Not medical advice. For research and education only, and not medical advice: this app designs restriction, Golden Gate and Gibson products from the sequences you supply; a designed product is not evidence that the reaction occurred, and nothing here predicts whether a reaction works, how well, or whether the product is expressed.
Ends
BspMI: ACCTGC, cut ACCTGCNNNN^NNNN_N, leaving a 4-base 5′ overhang whose bases are not fixed by the recognition site.
Methylation
Every one of these sites is cut in unmethylated DNA and in DNA from a dam and dcm methylating host, by the recorded dam and dcm sensitivities. Only dam and dcm are considered; CpG, EcoKI and other modifications are not modelled.
Source: McClelland M, Nelson M, Raschke E. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic Acids Res 1994;22:3640-3659 (doi 10.1093/nar/22.17.3640), Table I, read 6 October 2026.
The workspace draws a schematic gel of these fragments, not a prediction of where bands will run. Fragments under 100 bp are listed here but not drawn there, and fragments within 5 percent of each other are drawn as one band.
How this page was made
Computed when the site was built by the Drybench Plasmid engine, version 1.0.0, from /data/plasmid-map/examples/pegfp-n1.1.json (GenBank U55762.1, SHA-256 63918e4dcae6eed7f21a2d430f8b24a7172acc9a174e307d933550034189efde) and enzyme table version 1, /data/plasmid-map/enzymes.8829d35c83.json (2026-10-06). Every cut position and fragment length on this page was compared with Biopython 1.88 (REBASE release 404, recorded 2026-11-07); a disagreement stops the build.