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"withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H2", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H3", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H4", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H5", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H6", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H7", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H8", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H9", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H10", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H11", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H12", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Synthetic planned inputs; eight parallel rows are not measured biological replicates.", "Final per-row volumes 180/180/200 uL; original concentrations100/10/1 uM, changed1000/100/10 uM.", "System inventory includes BOTH stock reservoirs; changing selected stock does not change initial total55,000 nmol." ], "productionApplicability": "v1-transfer-and-mix", "title": "Three-column in-plate serial preparation" }, { "exactExpected": { "completedActions": 9, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "9/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "concentrate": "9/500000", "diluent": "81/500000" }, "volumeL": "9/50000", "withdrawalReserveL": "1/100000" }, "A10": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A10", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A11": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A11", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A12": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A12", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A2": { "amountsMol": { "demo-solute": "9/500000000" }, "capacityL": "3/10000", "compositionComplete": true, 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"withdrawalReserveL": "1/100000" }, "A6": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A6", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A7": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A7", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A8": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A8", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "A9": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A9", "originsL": {}, "volumeL": "0/1", 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"homogeneous": true, "id": "B12", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B2": { "amountsMol": { "demo-solute": "9/500000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": true, "id": "B2", "originsL": { "concentrate": "9/5000000", "diluent": "891/5000000" }, "volumeL": "9/50000", "withdrawalReserveL": "1/100000" }, "B3": { "amountsMol": { "demo-solute": "1/500000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/100000" }, "homogeneous": true, "id": "B3", "originsL": { "concentrate": "1/5000000", "diluent": "999/5000000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "B4": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B4", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B5": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B5", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B6": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B6", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B7": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B7", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B8": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B8", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B9": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B9", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C1": { "amountsMol": { "demo-solute": "9/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "C1", "originsL": { "concentrate": "9/500000", "diluent": "81/500000" }, "volumeL": "9/50000", "withdrawalReserveL": "1/100000" }, "C10": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C10", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C11": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C11", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C12": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C12", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C2": { "amountsMol": { "demo-solute": "9/500000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": true, "id": "C2", "originsL": { "concentrate": "9/5000000", "diluent": "891/5000000" }, "volumeL": "9/50000", "withdrawalReserveL": "1/100000" }, "C3": { "amountsMol": { "demo-solute": "1/500000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/100000" }, "homogeneous": true, "id": "C3", "originsL": { "concentrate": "1/5000000", "diluent": "999/5000000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "C4": { "amountsMol": { "demo-solute": "0/1" }, 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"amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H6", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H7", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H8", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H9", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H10", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H11", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H12", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Synthetic planned inputs; eight parallel rows are not measured biological replicates.", "Final per-row volumes 180/180/200 uL; original concentrations100/10/1 uM, changed1000/100/10 uM.", "System inventory includes BOTH stock reservoirs; changing selected stock does not change initial total55,000 nmol." ], "productionApplicability": "v1-transfer-and-mix", "title": "Three-column in-plate serial preparation" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "eight-at-once", "code": "source-reserve" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "D1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "E1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "F1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "G1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "H1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "H1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/500000" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/500" }, "volumeL": "1/500", "withdrawalReserveL": "1/2000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/500000" }, "volumeL": "1/500" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "eight-at-once", "code": "source-reserve" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "B1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "C1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "D1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "E1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "F1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "G1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "H1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "H1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 2e-06 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 0.002 }, "volumeL": 0.002, "withdrawalReserveL": 0.0005 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 2e-06 }, "volumeL": 0.002 } }, "status": "refused" }, "id": "aggregate-reservoir-refusal", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "eight-at-once-tip-1", "to": "A1", "volumeL": 0.0002 }, { "channel": 2, "from": "source", "tipId": "eight-at-once-tip-2", "to": "B1", "volumeL": 0.0002 }, { "channel": 3, "from": "source", "tipId": "eight-at-once-tip-3", "to": "C1", "volumeL": 0.0002 }, { "channel": 4, "from": "source", "tipId": "eight-at-once-tip-4", "to": "D1", "volumeL": 0.0002 }, { "channel": 5, "from": "source", "tipId": "eight-at-once-tip-5", "to": "E1", "volumeL": 0.0002 }, { "channel": 6, "from": "source", "tipId": "eight-at-once-tip-6", "to": "F1", "volumeL": 0.0002 }, { "channel": 7, "from": "source", "tipId": "eight-at-once-tip-7", "to": "G1", "volumeL": 0.0002 }, { "channel": 8, "from": "source", "tipId": "eight-at-once-tip-8", "to": "H1", "volumeL": 0.0002 } ], "id": "eight-at-once", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 2e-06 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.002 }, "volumeL": 0.002, "withdrawalReserveL": 0.0005 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Available1500 uL; aggregate request1600 uL. Every individual200 uL request looks affordable; no channel may commit." ], "productionApplicability": "v1-transfer-and-mix", "title": "Eight shared-source withdrawals cannot be checked individually" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "eighth-full", "code": "destination-capacity" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "B1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "C1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "D1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "E1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "F1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "G1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "H1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "H1", "originsL": { "H1": "29/100000" }, "volumeL": "29/100000", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/200000" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/200" }, "volumeL": "1/200", "withdrawalReserveL": "1/2000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/200000" }, "volumeL": "529/100000" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "eighth-full", "code": "destination-capacity" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "B1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "C1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "D1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "E1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "F1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "G1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "H1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "H1", "originsL": { "H1": 0.00029 }, "volumeL": 0.00029, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 5e-06 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 0.005 }, "volumeL": 0.005, "withdrawalReserveL": 0.0005 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 5e-06 }, "volumeL": 0.00529 } }, "status": "refused" }, "id": "eighth-destination-refusal", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "eighth-full-tip-1", "to": "A1", "volumeL": 2e-05 }, { "channel": 2, "from": "source", "tipId": "eighth-full-tip-2", "to": "B1", "volumeL": 2e-05 }, { "channel": 3, "from": "source", "tipId": "eighth-full-tip-3", "to": "C1", "volumeL": 2e-05 }, { "channel": 4, "from": "source", "tipId": "eighth-full-tip-4", "to": "D1", "volumeL": 2e-05 }, { "channel": 5, "from": "source", "tipId": "eighth-full-tip-5", "to": "E1", "volumeL": 2e-05 }, { "channel": 6, "from": "source", "tipId": "eighth-full-tip-6", "to": "F1", "volumeL": 2e-05 }, { "channel": 7, "from": "source", "tipId": "eighth-full-tip-7", "to": "G1", "volumeL": 2e-05 }, { "channel": 8, "from": "source", "tipId": "eighth-full-tip-8", "to": "H1", "volumeL": 2e-05 } ], "id": "eighth-full", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 5e-06 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.005 }, "volumeL": 0.005, "withdrawalReserveL": 0.0005 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "B1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "C1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "D1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "E1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "F1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "G1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "H1", "originsL": { "H1": 0.00029 }, "volumeL": 0.00029, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "H1 would reach310 uL above300 uL; A1:G1 must remain empty and source unchanged." ], "productionApplicability": "v1-transfer-and-mix", "title": "Eighth channel over capacity leaves all channels unchanged" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "unmixed", "code": "source-unmixed" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/10000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": false, "id": "source", "originsL": { "source": "1/10000" }, "volumeL": "1/10000", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "unmixed", "code": "source-unmixed" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": false, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, "status": "refused" }, "id": "unmixed-source-refusal", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "unmixed-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "unmixed", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": false, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Known amount and bulk average do not justify the aspirated fraction without the ideal-homogeneity declaration." ], "productionApplicability": "v1-transfer-and-mix", "title": "Bulk average cannot determine an unmixed aliquot" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 1, "refusal": { "actionId": "unmixed-next", "code": "source-unmixed" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": false, "id": "A1", "originsL": { "A1": "9/50000", "source": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "A2": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A2", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "seed" ], "potentialContactRoutes": [], "tips": { "seed-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "seed-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "7/25000" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 1, "refusal": { "actionId": "unmixed-next", "code": "source-unmixed" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0001 }, "homogeneous": false, "id": "A1", "originsL": { "A1": 0.00018, "source": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "A2": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A2", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "seed" ], "potentialContactRoutes": [], "tips": { "seed-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "seed-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.00028 } }, "status": "refused" }, "id": "unmixed-prefix-refusal", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "seed-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "seed", "kind": "transfer" }, { "channels": [ { "channel": 1, "from": "A1", "tipId": "unmixed-next-tip-1", "to": "A2", "volumeL": 2e-05 } ], "id": "unmixed-next", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": { "A1": 0.00018 }, "volumeL": 0.00018, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A2", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Seed is accepted: source80 uL,A1 bulk average100 uM at200 uL. A1 must remain unchanged when its next transfer is refused." ], "productionApplicability": "v1-transfer-and-mix", "title": "Keep accepted prefix and refuse next unmixed aspiration" }, { "exactExpected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "A1": "9/50000", "unknown-stock": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "unknown-stock": { "amountsMol": { "demo-solute": null }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unknown-stock", "originsL": { "unknown-stock": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "unknown-known-volume", "mix" ], "potentialContactRoutes": [], "tips": { "unknown-known-volume-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unknown-stock", "A1" ], "discarded": false, "homogeneous": true, "id": "unknown-known-volume-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": "7/25000" } }, "status": "accepted" }, "expected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "A1": 0.00018, "unknown-stock": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "unknown-stock": { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unknown-stock", "originsL": { "unknown-stock": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "unknown-known-volume", "mix" ], "potentialContactRoutes": [], "tips": { "unknown-known-volume-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unknown-stock", "A1" ], "discarded": false, "homogeneous": true, "id": "unknown-known-volume-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": 0.00028 } }, "status": "accepted" }, "id": "unknown-analyte", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "unknown-stock", "tipId": "unknown-known-volume-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "unknown-known-volume", "kind": "transfer" }, { "containers": [ "A1" ], "id": "mix", "kind": "declareHomogeneous" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "unknown-stock", "originsL": { "unknown-stock": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": { "A1": 0.00018 }, "volumeL": 0.00018, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "A1 volume200 uL and origin volumes20/180 uL are known. Analyte amount and concentration are null; no carried old value and no zero substitution." ], "productionApplicability": "v1-transfer-and-mix", "title": "Known volume and ancestry with explicitly unknown analyte amount" }, { "exactExpected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": "3/10000", "compositionComplete": false, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "A1": "9/50000", "unclassified": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "unclassified": { "amountsMol": {}, "capacityL": "3/10000", "compositionComplete": false, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unclassified", "originsL": { "unclassified": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "incomplete", "mix" ], "potentialContactRoutes": [], "tips": { "incomplete-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unclassified", "A1" ], "discarded": false, "homogeneous": true, "id": "incomplete-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": "7/25000" } }, "status": "accepted" }, "expected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": false, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "A1": 0.00018, "unclassified": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "unclassified": { "amountsMol": {}, "capacityL": 0.0003, "compositionComplete": false, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unclassified", "originsL": { "unclassified": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "incomplete", "mix" ], "potentialContactRoutes": [], "tips": { "incomplete-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unclassified", "A1" ], "discarded": false, "homogeneous": true, "id": "incomplete-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": 0.00028 } }, "status": "accepted" }, "id": "incomplete-composition", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "unclassified", "tipId": "incomplete-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "incomplete", "kind": "transfer" }, { "containers": [ "A1" ], "id": "mix", "kind": "declareHomogeneous" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": {}, "capacityL": 0.0003, "compositionComplete": false, "homogeneous": true, "id": "unclassified", "originsL": { "unclassified": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": { "A1": 0.00018 }, "volumeL": 0.00018, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Omitting a named analyte from an incomplete composition does not establish absence. A1 analyte concentration must be unavailable." ], "productionApplicability": "v1-transfer-and-mix", "title": "Absent analyte key in incomplete composition is unknown" }, { "exactExpected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "1/50000" }, "volumeL": "1/50000", "withdrawalReserveL": "1/100000" }, "A2": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "A2", "originsL": { "diluent": "1/50000" }, "volumeL": "1/50000", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "49/50000" }, "volumeL": "49/50000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "touch-source", "reenter-diluent" ], "potentialContactRoutes": [ { "actionId": "reenter-diluent", "from": "source", "tipId": "T1", "to": "diluent" }, { "actionId": "reenter-diluent", "from": "A1", "tipId": "T1", "to": "diluent" } ], "tips": { "T1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1", "diluent", "A2" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } }, "status": "accepted" }, "expected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 2e-05 }, "volumeL": 2e-05, "withdrawalReserveL": 1e-05 }, "A2": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "A2", "originsL": { "diluent": 2e-05 }, "volumeL": 2e-05, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.00098 }, "volumeL": 0.00098, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "touch-source", "reenter-diluent" ], "potentialContactRoutes": [ { "actionId": "reenter-diluent", "from": "source", "tipId": "T1", "to": "diluent" }, { "actionId": "reenter-diluent", "from": "A1", "tipId": "T1", "to": "diluent" } ], "tips": { "T1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1", "diluent", "A2" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } }, "status": "accepted" }, "id": "tip-reuse-no-quantified-carryover", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "T1", "to": "A1", "volumeL": 2e-05 } ], "id": "touch-source", "kind": "transfer" }, { "channels": [ { "channel": 1, "from": "diluent", "tipId": "T1", "to": "A2", "volumeL": 2e-05 } ], "id": "reenter-diluent", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.001 }, "volumeL": 0.001, "withdrawalReserveL": 0.0005 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A2", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "T1 previously contacted source andA1, then re-enters diluent: both prior-contact routes are recorded. A2 receives0 mol nominal analyte, not a guessed contamination amount. Zero nominal carryover is an assumption, not proof of a clean tip." ], "productionApplicability": "v1-transfer-and-mix", "title": "Re-entry contact routes do not add nominal solute" }, { "exactExpected": { "completedActions": 0, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "0/1" }, "volumeL": "0/1" } }, "status": "accepted" }, "expected": { "completedActions": 0, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 0.0 }, "volumeL": 0.0 } }, "status": "accepted" }, "id": "empty-well", "input": { "actions": [], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "0 mol/0 L is not a0 M control." ], "productionApplicability": "v1-transfer-and-mix", "title": "Empty amount zero but concentration undefined" }, { "exactExpected": { "checkpoints": [ { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/10000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/10000" }, "volumeL": "1/10000", "withdrawalReserveL": "0/1" }, "waste": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" }, "waste": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "aspirate" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": "1/10000000" }, "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "contacts": [ "source" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": { "source": "1/10000" }, "volumeL": "1/10000" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "3/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "3/50000" }, "volumeL": "3/50000", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" }, "waste": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "aspirate", "dispense-part" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": "1/25000000" }, "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": { "source": "1/25000" }, "volumeL": "1/25000" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "3/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "3/50000" }, "volumeL": "3/50000", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" }, "waste": { "amountsMol": { "demo-solute": "1/25000000" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "waste", "originsL": { "source": "1/25000" }, "volumeL": "1/25000", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "aspirate", "dispense-part", "discard" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": true, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } } ], "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "3/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "3/50000" }, "volumeL": "3/50000", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" }, "waste": { "amountsMol": { "demo-solute": "1/25000000" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "waste", "originsL": { "source": "1/25000" }, "volumeL": "1/25000", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "aspirate", "dispense-part", "discard" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": true, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, "status": "accepted" }, "expected": { "checkpoints": [ { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 0.0 }, "waste": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 }, "waste": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "aspirate" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": 1e-07 }, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "contacts": [ "source" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": { "source": 0.0001 }, "volumeL": 0.0001 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 6e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 6e-05 }, "volumeL": 6e-05, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 }, "waste": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "aspirate", "dispense-part" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": 4e-08 }, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "T1", "originsL": { "source": 4e-05 }, "volumeL": 4e-05 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 6e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 6e-05 }, "volumeL": 6e-05, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 }, "waste": { "amountsMol": { "demo-solute": 4e-08 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "waste", "originsL": { "source": 4e-05 }, "volumeL": 4e-05, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "aspirate", "dispense-part", "discard" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": true, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } } ], "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 6e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 6e-05 }, "volumeL": 6e-05, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "source", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 }, "waste": { "amountsMol": { "demo-solute": 4e-08 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "waste", "originsL": { "source": 4e-05 }, "volumeL": 4e-05, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "aspirate", "dispense-part", "discard" ], "potentialContactRoutes": [], "tips": { "T1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": true, "homogeneous": true, "id": "T1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, "status": "accepted" }, "id": "partial-tip-waste", "input": { "actions": [ { "from": "source", "id": "aspirate", "kind": "aspirate", "tipId": "T1", "volumeL": 0.0001 }, { "id": "dispense-part", "kind": "dispense", "tipId": "T1", "to": "A1", "volumeL": 6e-05 }, { "id": "discard", "kind": "discardTip", "tipId": "T1", "waste": "waste" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 0.0 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "After aspiration tip100 uL/100 nmol; after dispense tip40 uL/40 nmol andA1 60 uL/60 nmol; discard moves40 uL/40 nmol to waste.", "Production v1 supports complete nominal transfers only. Its reader must refuse these primitive actions; this reference does not require exposing them." ], "productionApplicability": "future-primitives-not-supported-by-v1", "title": "Retained tip contents move into explicit waste" }, { "exactExpected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "1/50000" }, "volumeL": "1/50000", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "3/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "3/50000" }, "volumeL": "3/50000", "withdrawalReserveL": "1/100000" }, "waste": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "waste", "originsL": { "source": "1/50000" }, "volumeL": "1/50000", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "use", "discard-liquid" ], "potentialContactRoutes": [], "tips": { "discard-liquid-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "waste" ], "discarded": false, "homogeneous": true, "id": "discard-liquid-tip-1", "originsL": {}, "volumeL": "0/1" }, "use-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "use-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, "status": "accepted" }, "expected": { "completedActions": 2, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 2e-05 }, "volumeL": 2e-05, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 6e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 6e-05 }, "volumeL": 6e-05, "withdrawalReserveL": 1e-05 }, "waste": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "waste", "originsL": { "source": 2e-05 }, "volumeL": 2e-05, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "use", "discard-liquid" ], "potentialContactRoutes": [], "tips": { "discard-liquid-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "waste" ], "discarded": false, "homogeneous": true, "id": "discard-liquid-tip-1", "originsL": {}, "volumeL": 0.0 }, "use-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "use-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, "status": "accepted" }, "id": "full-transfer-waste", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "use-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "use", "kind": "transfer" }, { "channels": [ { "channel": 1, "from": "source", "tipId": "discard-liquid-tip-1", "to": "waste", "volumeL": 2e-05 } ], "id": "discard-liquid", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "waste", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Source60 uL/60 nmol,A1 20 uL/20 nmol,waste20 uL/20 nmol; system100 uL/100 nmol. Liquid in waste remains in conservation accounting." ], "productionApplicability": "v1-transfer-and-mix", "title": "V1 waste is an explicit destination of a complete transfer" }, { "exactExpected": { "completedActions": 1, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "unknown-stock": "1/10000" }, "volumeL": "1/10000", "withdrawalReserveL": "1/100000" }, "unknown-stock": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unknown-stock", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "0/1" } }, "executedActionIds": [ "empty-source" ], "potentialContactRoutes": [], "tips": { "empty-source-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unknown-stock", "A1" ], "discarded": false, "homogeneous": true, "id": "empty-source-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": "1/10000" } }, "status": "accepted" }, "expected": { "completedActions": 1, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": { "unknown-stock": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, "unknown-stock": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "unknown-stock", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 0.0 } }, "executedActionIds": [ "empty-source" ], "potentialContactRoutes": [], "tips": { "empty-source-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "unknown-stock", "A1" ], "discarded": false, "homogeneous": true, "id": "empty-source-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": null }, "volumeL": 0.0001 } }, "status": "accepted" }, "id": "unknown-source-emptied", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "unknown-stock", "tipId": "empty-source-tip-1", "to": "A1", "volumeL": 0.0001 } ], "id": "empty-source", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": null }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "unknown-stock", "originsL": { "unknown-stock": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 0.0 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Source ends0 L and0 mol with undefined concentration. Destination100 uL has unknown analyte amount/concentration. Do not retain an unknown amount in an empty source under the no-residue model." ], "productionApplicability": "v1-transfer-and-mix", "title": "No-residue model empties an unknown-composition source completely" }, { "exactExpected": { "checkpoints": [ { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "1/1000" }, "volumeL": "1/1000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/10000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/10000" }, "volumeL": "1/10000", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "A1", "originsL": { "source": "1/50000" }, "volumeL": "1/50000", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "1/1000" }, "volumeL": "1/1000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "first" ], "potentialContactRoutes": [], "tips": { "first-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": false, "id": "A1", "originsL": { "diluent": "9/50000", "source": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "41/50000" }, "volumeL": "41/50000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "first", "dilute" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": "0/1" }, "first-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } }, { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": true, "id": "A1", "originsL": { "diluent": "9/50000", "source": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "41/50000" }, "volumeL": "41/50000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "first", "dilute", "mix" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": "0/1" }, "first-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } } ], "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "1/50000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/10000" }, "homogeneous": true, "id": "A1", "originsL": { "diluent": "9/50000", "source": "1/50000" }, "volumeL": "1/5000", "withdrawalReserveL": "1/100000" }, "diluent": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "1/40", "compositionComplete": true, "concentrationsM": { "demo-solute": "0/1" }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": "41/50000" }, "volumeL": "41/50000", "withdrawalReserveL": "1/2000" }, "source": { "amountsMol": { "demo-solute": "1/12500000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "first", "dilute", "mix" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": "0/1" }, "first-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "11/10000" } }, "status": "accepted" }, "expected": { "checkpoints": [ { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.001 }, "volumeL": 0.001, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "A1", "originsL": { "source": 2e-05 }, "volumeL": 2e-05, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.001 }, "volumeL": 0.001, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "first" ], "potentialContactRoutes": [], "tips": { "first-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0001 }, "homogeneous": false, "id": "A1", "originsL": { "diluent": 0.00018, "source": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.00082 }, "volumeL": 0.00082, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "first", "dilute" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": 0.0 }, "first-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } }, { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0001 }, "homogeneous": true, "id": "A1", "originsL": { "diluent": 0.00018, "source": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.00082 }, "volumeL": 0.00082, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "first", "dilute", "mix" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": 0.0 }, "first-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } } ], "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 2e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0001 }, "homogeneous": true, "id": "A1", "originsL": { "diluent": 0.00018, "source": 2e-05 }, "volumeL": 0.0002, "withdrawalReserveL": 1e-05 }, "diluent": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0 }, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.00082 }, "volumeL": 0.00082, "withdrawalReserveL": 0.0005 }, "source": { "amountsMol": { "demo-solute": 8e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "first", "dilute", "mix" ], "potentialContactRoutes": [], "tips": { "dilute-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "diluent", "A1" ], "discarded": false, "homogeneous": true, "id": "dilute-tip-1", "originsL": {}, "volumeL": 0.0 }, "first-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "contacts": [ "source", "A1" ], "discarded": false, "homogeneous": true, "id": "first-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0011 } }, "status": "accepted" }, "id": "replay-prefixes", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "first-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "first", "kind": "transfer" }, { "channels": [ { "channel": 1, "from": "diluent", "tipId": "dilute-tip-1", "to": "A1", "volumeL": 0.00018 } ], "id": "dilute", "kind": "transfer" }, { "containers": [ "A1" ], "id": "mix", "kind": "declareHomogeneous" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.025, "compositionComplete": true, "homogeneous": true, "id": "diluent", "originsL": { "diluent": 0.001 }, "volumeL": 0.001, "withdrawalReserveL": 0.0005 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "Initial emptyA1; first20 uL at1 mM; afterdiluent200 uL bulkaverage100 uM/unmixed; finalsamequantities/homogeneous. Replay from initial reproduces each prefix; no draw-timing dependence." ], "productionApplicability": "v1-transfer-and-mix", "title": "Replay checkpoints are exact accepted-prefix states" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "below-min", "code": "pipette-range" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/10000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/10000" }, "volumeL": "1/10000", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/10000000" }, "volumeL": "1/10000" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "below-min", "code": "pipette-range" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 1e-07 }, "volumeL": 0.0001 } }, "status": "refused" }, "id": "below-pipette-minimum", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "below-min-tip-1", "to": "A1", "volumeL": 1.9e-05 } ], "id": "below-min", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "19 uL is outside declared20–200 uL." ], "productionApplicability": "v1-transfer-and-mix", "title": "No rounding into instrument range" }, { "exactExpected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "reserve", "code": "source-reserve" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": "0/1", "withdrawalReserveL": "1/100000" }, "source": { "amountsMol": { "demo-solute": "1/40000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000" }, "homogeneous": true, "id": "source", "originsL": { "source": "1/40000" }, "volumeL": "1/40000", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": "1/40000000" }, "volumeL": "1/40000" } }, "status": "refused" }, "expected": { "atomicNoPartialMutation": true, "completedActions": 0, "refusal": { "actionId": "reserve", "code": "source-reserve" }, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": null }, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 }, "source": { "amountsMol": { "demo-solute": 2.5e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001 }, "homogeneous": true, "id": "source", "originsL": { "source": 2.5e-05 }, "volumeL": 2.5e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [], "potentialContactRoutes": [], "tips": {}, "totals": { "amountsMol": { "demo-solute": 2.5e-08 }, "volumeL": 2.5e-05 } }, "status": "refused" }, "id": "single-source-reserve", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "source", "tipId": "reserve-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "reserve", "kind": "transfer" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 2.5e-08 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "source", "originsL": { "source": 2.5e-05 }, "volumeL": 2.5e-05, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" } ], "tips": {} }, "notes": [ "25−20=5 uL would remain below10 uL reserve." ], "productionApplicability": "v1-transfer-and-mix", "title": "Reserve is not extra liquid available to transfer" }, { "exactExpected": { "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": "3/50000000", "marker": "1/100000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "3/2000", "marker": "1/4000" }, "homogeneous": true, "id": "A1", "originsL": { "lot-a": "1/50000", "lot-b": "1/50000" }, "volumeL": "1/25000", "withdrawalReserveL": "1/100000" }, "lot-a": { "amountsMol": { "demo-solute": "1/12500000", "marker": "1/25000000" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/1000", "marker": "1/2000" }, "homogeneous": true, "id": "lot-a", "originsL": { "lot-a": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" }, "lot-b": { "amountsMol": { "demo-solute": "1/6250000", "marker": "0/1" }, "capacityL": "3/10000", "compositionComplete": true, "concentrationsM": { "demo-solute": "1/500", "marker": "0/1" }, "homogeneous": true, "id": "lot-b", "originsL": { "lot-b": "1/12500" }, "volumeL": "1/12500", "withdrawalReserveL": "1/100000" } }, "executedActionIds": [ "lot-a", "lot-b", "mix" ], "potentialContactRoutes": [], "tips": { "lot-a-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null, "marker": null }, "contacts": [ "lot-a", "A1" ], "discarded": false, "homogeneous": true, "id": "lot-a-tip-1", "originsL": {}, "volumeL": "0/1" }, "lot-b-tip-1": { "amountsMol": { "demo-solute": "0/1" }, "compositionComplete": true, "concentrationsM": { "demo-solute": null, "marker": null }, "contacts": [ "lot-b", "A1" ], "discarded": false, "homogeneous": true, "id": "lot-b-tip-1", "originsL": {}, "volumeL": "0/1" } }, "totals": { "amountsMol": { "demo-solute": "3/10000000", "marker": "1/20000000" }, "volumeL": "1/5000" } }, "status": "accepted" }, "expected": { "completedActions": 3, "state": { "containers": { "A1": { "amountsMol": { "demo-solute": 6e-08, "marker": 1e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.0015, "marker": 0.00025 }, "homogeneous": true, "id": "A1", "originsL": { "lot-a": 2e-05, "lot-b": 2e-05 }, "volumeL": 4e-05, "withdrawalReserveL": 1e-05 }, "lot-a": { "amountsMol": { "demo-solute": 8e-08, "marker": 4e-08 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.001, "marker": 0.0005 }, "homogeneous": true, "id": "lot-a", "originsL": { "lot-a": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 }, "lot-b": { "amountsMol": { "demo-solute": 1.6e-07, "marker": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "concentrationsM": { "demo-solute": 0.002, "marker": 0.0 }, "homogeneous": true, "id": "lot-b", "originsL": { "lot-b": 8e-05 }, "volumeL": 8e-05, "withdrawalReserveL": 1e-05 } }, "executedActionIds": [ "lot-a", "lot-b", "mix" ], "potentialContactRoutes": [], "tips": { "lot-a-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null, "marker": null }, "contacts": [ "lot-a", "A1" ], "discarded": false, "homogeneous": true, "id": "lot-a-tip-1", "originsL": {}, "volumeL": 0.0 }, "lot-b-tip-1": { "amountsMol": { "demo-solute": 0.0 }, "compositionComplete": true, "concentrationsM": { "demo-solute": null, "marker": null }, "contacts": [ "lot-b", "A1" ], "discarded": false, "homogeneous": true, "id": "lot-b-tip-1", "originsL": {}, "volumeL": 0.0 } }, "totals": { "amountsMol": { "demo-solute": 3e-07, "marker": 5e-08 }, "volumeL": 0.0002 } }, "status": "accepted" }, "id": "identity-versus-origin", "input": { "actions": [ { "channels": [ { "channel": 1, "from": "lot-a", "tipId": "lot-a-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "lot-a", "kind": "transfer" }, { "channels": [ { "channel": 1, "from": "lot-b", "tipId": "lot-b-tip-1", "to": "A1", "volumeL": 2e-05 } ], "id": "lot-b", "kind": "transfer" }, { "containers": [ "A1" ], "id": "mix", "kind": "declareHomogeneous" } ], "assumptions": { "additiveVolumes": true, "completeNominalDispense": true, "idealMixingDeclarations": true, "quantifiedCarryover": false }, "containers": [ { "amountsMol": { "demo-solute": 1e-07, "marker": 5e-08 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "lot-a", "originsL": { "lot-a": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 2e-07, "marker": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "lot-b", "originsL": { "lot-b": 0.0001 }, "volumeL": 0.0001, "withdrawalReserveL": 1e-05 }, { "amountsMol": { "demo-solute": 0.0 }, "capacityL": 0.0003, "compositionComplete": true, "homogeneous": true, "id": "A1", "originsL": {}, "volumeL": 0.0, "withdrawalReserveL": 1e-05 } ], "instrument": { "maximumL": 0.0002, "minimumL": 2e-05, "supportedCommandChannelCounts": [ 1, 8 ], "tipCapacityL": 0.0002 }, "materials": [ { "basis": "mol", "id": "demo-solute", "label": "Synthetic demo solute" }, { "basis": "mol", "id": "marker", "label": "Synthetic second species" } ], "tips": {} }, "notes": [ "A1:40 uL,60 nmol demo-solute,10 nmol marker,20 uL from each lot. Concentrations1.5 mM and0.25 mM respectively." ], "productionApplicability": "v1-transfer-and-mix", "title": "Same analyte identity combines while source lots remain distinct" } ], "schema": "drybench-wet-lab-independent-reference", "sourceImports": "None: no production or prototype engine imports.", "status": "Synthetic planned scenarios; independent mathematical references, not instrument calibration.", "tolerance": { "amountMolAbsolute": 1e-21, "concentrationMAbsolute": 1e-15, "meaning": "Numerical comparison thresholds, not physical accuracy or uncertainty.", "relative": 1e-12, "volumeLAbsolute": 1e-15 }, "units": { "amount": "mol", "concentration": "mol/L", "volume": "L" }, "version": "1.0.0" }