{ "about": { "generatedBy": "pipeline/plasmid-map/reference.py", "scriptSha256": "d268790409489fe6bb1d821bc5625a78d7d3c51fb2559a5de6629c33fbdb7f09", "recordedOn": "2026-10-17", "oracle": "Biopython 1.88", "oracleLicence": "Biopython Licence Agreement or BSD-3-Clause", "text": "The correct, empty and reversed construct trio (brief section 4.3 (5)): EGFP's coding sequence (U55762.1 679..1398, 720 bp) in pUC19's SmaI site in either orientation, and pUC19 closed on itself. Fragments from Biopython's search(linear=False); bands read by the schematic gel's rules (fragments under 100 bp not visible; a fragment joins a band when within 5 percent, inclusive, of the band's largest fragment (gel rules 2)); two constructs are told apart when their band counts differ or any pair of bands, largest first, differs by more than 5 percent. The pool: enzymes cutting the correct construct 1 to 6 times and pairs of them with at most 8 cuts together; a digest leaving any construct uncut is not offered.", "minVisibleBp": 100, "resolveFraction": "0.05", "poolMaxCuts": 6, "pairMaxCuts": 8, "gelRulesVersion": "2.0.0", "changeAgainstV1": "43 of 4,157 digests of the EGFP-in-pUC19 trio change their bands under gel rule 2.0.0; no picker verdict on that trio changes. The digests that tell all three constructs apart are the same set under both rules." }, "constructs": { "correct": { "length": 3406, "sha256": "ed67d715de9101ca2b8727bcd40cbb45f77bb785400d94b1feadd1c3d325548d" }, "empty": { "length": 2686, "sha256": "8a205af08518dae1b0045c7b098986dca18824ef654e75233603602ab89cad04" }, "reversed": { "length": 3406, "sha256": "7465f438b0b99e7d105911d06b915549c6b4e713f039b52c2362fbceb1194d46" } }, "insert": { "accession": "U55762.1", "start": 679, "end": 1398, "length": 720 }, "smaICut": 414, "pool": [ "AatII", "Acc65I", "AccI", "AclI", "AcuI", "AflIII", "AhdI", "AleI", "AlwNI", "ApaLI", "ApoI", "AseI", "AvaII", "BaeGI", "BamHI", "BanI", "BanII", "BccI", "BciVI", "BfaI", "BglI", "BmrI", "BpmI", "Bpu10I", "BpuEI", "BsaHI", "BsaI", "BsaWI", "BseRI", "BseYI", "BsgI", "BsiEI", "BsiHKAI", "BsmAI", "BsmBI", "BspHI", "BspMI", "BsrBI", "BsrDI", "BsrFI", "BsrGI", "BssSI", "BstAPI", "BtgI", "BtgZI", "BtsI", "CviQI", "DraI", "DrdI", "EaeI", "EarI", "EciI", "Eco53kI", "EcoO109I", "EcoRI", "FauI", "FspI", "HaeII", "HgaI", "HincII", "HindIII", "HinfI", "KasI", "KpnI", "MlyI", "MmeI", "MspA1I", "NarI", "NcoI", "NdeI", "NmeAIII", "NspI", "PciI", "PleI", "PluTI", "PstI", "PvuI", "PvuII", "RsaI", "SacI", "SalI", "SapI", "SbfI", "ScaI", "SfcI", "SfoI", "SmlI", "SphI", "SspI", "StyI", "TfiI", "Tsp45I", "XbaI", "XmnI", "ZraI" ], "cases": [ { "enzymes": [ "AatII" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "Acc65I" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AccI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AclI" ], "fragments": { "correct": [ 3033, 373 ], "empty": [ 2313, 373 ], "reversed": [ 3033, 373 ] }, "bands": { "correct": [ [ 3033 ], [ 373 ] ], "empty": [ [ 2313 ], [ 373 ] ], "reversed": [ [ 3033 ], [ 373 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AcuI" ], "fragments": { "correct": [ 1263, 1012, 888, 199, 44 ], "empty": [ 1674, 1012 ], "reversed": [ 1092, 1059, 1012, 199, 44 ] }, "bands": { "correct": [ [ 1263 ], [ 1012 ], [ 888 ], [ 199 ] ], "empty": [ [ 1674 ], [ 1012 ] ], "reversed": [ [ 1092, 1059 ], [ 1012 ], [ 199 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "AflIII" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AhdI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AlwNI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "ApaLI" ], "fragments": { "correct": [ 1663, 1246, 497 ], "empty": [ 1246, 943, 497 ], "reversed": [ 1663, 1246, 497 ] }, "bands": { "correct": [ [ 1663 ], [ 1246 ], [ 497 ] ], "empty": [ [ 1246 ], [ 943 ], [ 497 ] ], "reversed": [ [ 1663 ], [ 1246 ], [ 497 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "ApoI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AseI" ], "fragments": { "correct": [ 2112, 1235, 59 ], "empty": [ 1392, 1235, 59 ], "reversed": [ 2112, 1235, 59 ] }, "bands": { "correct": [ [ 2112 ], [ 1235 ] ], "empty": [ [ 1392 ], [ 1235 ] ], "reversed": [ [ 2112 ], [ 1235 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "AvaII" ], "fragments": { "correct": [ 1698, 1486, 222 ], "empty": [ 2464, 222 ], "reversed": [ 2083, 1101, 222 ] }, "bands": { "correct": [ [ 1698 ], [ 1486 ], [ 222 ] ], "empty": [ [ 2464 ], [ 222 ] ], "reversed": [ [ 2083 ], [ 1101 ], [ 222 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BaeGI" ], "fragments": { "correct": [ 1246, 1011, 497, 274, 249, 129 ], "empty": [ 1246, 943, 497 ], "reversed": [ 1246, 747, 538, 497, 249, 129 ] }, "bands": { "correct": [ [ 1246 ], [ 1011 ], [ 497 ], [ 274 ], [ 249 ], [ 129 ] ], "empty": [ [ 1246 ], [ 943 ], [ 497 ] ], "reversed": [ [ 1246 ], [ 747 ], [ 538 ], [ 497 ], [ 249 ], [ 129 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BamHI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BanI" ], "fragments": { "correct": [ 1274, 1097, 820, 173, 42 ], "empty": [ 1274, 1097, 173, 142 ], "reversed": [ 1274, 1097, 686, 176, 173 ] }, "bands": { "correct": [ [ 1274 ], [ 1097 ], [ 820 ], [ 173 ] ], "empty": [ [ 1274 ], [ 1097 ], [ 173 ], [ 142 ] ], "reversed": [ [ 1274 ], [ 1097 ], [ 686 ], [ 176, 173 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BanII" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BccI" ], "fragments": { "correct": [ 1478, 995, 287, 270, 252, 124 ], "empty": [ 2275, 287, 124 ], "reversed": [ 1379, 1094, 287, 270, 252, 124 ] }, "bands": { "correct": [ [ 1478 ], [ 995 ], [ 287 ], [ 270 ], [ 252 ], [ 124 ] ], "empty": [ [ 2275 ], [ 287 ], [ 124 ] ], "reversed": [ [ 1379 ], [ 1094 ], [ 287 ], [ 270 ], [ 252 ], [ 124 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BciVI" ], "fragments": { "correct": [ 1879, 1527 ], "empty": [ 1527, 1159 ], "reversed": [ 1879, 1527 ] }, "bands": { "correct": [ [ 1879 ], [ 1527 ] ], "empty": [ [ 1527 ], [ 1159 ] ], "reversed": [ [ 1879 ], [ 1527 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BfaI" ], "fragments": { "correct": [ 1941, 877, 335, 253 ], "empty": [ 1221, 877, 335, 253 ], "reversed": [ 1941, 877, 335, 253 ] }, "bands": { "correct": [ [ 1941 ], [ 877 ], [ 335 ], [ 253 ] ], "empty": [ [ 1221 ], [ 877 ], [ 335 ], [ 253 ] ], "reversed": [ [ 1941 ], [ 877 ], [ 335 ], [ 253 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BglI" ], "fragments": { "correct": [ 2288, 1118 ], "empty": [ 1568, 1118 ], "reversed": [ 2288, 1118 ] }, "bands": { "correct": [ [ 2288 ], [ 1118 ] ], "empty": [ [ 1568 ], [ 1118 ] ], "reversed": [ [ 2288 ], [ 1118 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BmrI" ], "fragments": { "correct": [ 1439, 1306, 661 ], "empty": [ 1380, 1306 ], "reversed": [ 1930, 1306, 170 ] }, "bands": { "correct": [ [ 1439 ], [ 1306 ], [ 661 ] ], "empty": [ [ 1380 ], [ 1306 ] ], "reversed": [ [ 1930 ], [ 1306 ], [ 170 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BpmI" ], "fragments": { "correct": [ 1776, 1390, 240 ], "empty": [ 2686 ], "reversed": [ 1798, 1368, 240 ] }, "bands": { "correct": [ [ 1776 ], [ 1390 ], [ 240 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 1798 ], [ 1368 ], [ 240 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BpuEI" ], "fragments": { "correct": [ 1999, 868, 298, 241 ], "empty": [ 1279, 868, 298, 241 ], "reversed": [ 1999, 868, 298, 241 ] }, "bands": { "correct": [ [ 1999 ], [ 868 ], [ 298 ], [ 241 ] ], "empty": [ [ 1279 ], [ 868 ], [ 298 ], [ 241 ] ], "reversed": [ [ 1999 ], [ 868 ], [ 298 ], [ 241 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsaHI" ], "fragments": { "correct": [ 2720, 382, 304 ], "empty": [ 2000, 382, 304 ], "reversed": [ 2720, 382, 304 ] }, "bands": { "correct": [ [ 2720 ], [ 382 ], [ 304 ] ], "empty": [ [ 2000 ], [ 382 ], [ 304 ] ], "reversed": [ [ 2720 ], [ 382 ], [ 304 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsaI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsaWI" ], "fragments": { "correct": [ 2428, 831, 147 ], "empty": [ 1708, 831, 147 ], "reversed": [ 2428, 831, 147 ] }, "bands": { "correct": [ [ 2428 ], [ 831 ], [ 147 ] ], "empty": [ [ 1708 ], [ 831 ], [ 147 ] ], "reversed": [ [ 2428 ], [ 831 ], [ 147 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BseYI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsiEI" ], "fragments": { "correct": [ 1163, 923, 747, 424, 149 ], "empty": [ 923, 747, 443, 424, 149 ], "reversed": [ 1163, 923, 747, 424, 149 ] }, "bands": { "correct": [ [ 1163 ], [ 923 ], [ 747 ], [ 424 ], [ 149 ] ], "empty": [ [ 923 ], [ 747 ], [ 443, 424 ], [ 149 ] ], "reversed": [ [ 1163 ], [ 923 ], [ 747 ], [ 424 ], [ 149 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsiHKAI" ], "fragments": { "correct": [ 1161, 820, 618, 497, 225, 85 ], "empty": [ 1161, 718, 497, 225, 85 ], "reversed": [ 1316, 1161, 497, 225, 122, 85 ] }, "bands": { "correct": [ [ 1161 ], [ 820 ], [ 618 ], [ 497 ], [ 225 ] ], "empty": [ [ 1161 ], [ 718 ], [ 497 ], [ 225 ] ], "reversed": [ [ 1316 ], [ 1161 ], [ 497 ], [ 225 ], [ 122 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BsmAI" ], "fragments": { "correct": [ 2435, 776, 153, 42 ], "empty": [ 1715, 776, 153, 42 ], "reversed": [ 2435, 776, 153, 42 ] }, "bands": { "correct": [ [ 2435 ], [ 776 ], [ 153 ] ], "empty": [ [ 1715 ], [ 776 ], [ 153 ] ], "reversed": [ [ 2435 ], [ 776 ], [ 153 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsmBI" ], "fragments": { "correct": [ 3364, 42 ], "empty": [ 2644, 42 ], "reversed": [ 3364, 42 ] }, "bands": { "correct": [ [ 3364 ] ], "empty": [ [ 2644 ] ], "reversed": [ [ 3364 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BspHI" ], "fragments": { "correct": [ 2293, 1008, 105 ], "empty": [ 1573, 1008, 105 ], "reversed": [ 2293, 1008, 105 ] }, "bands": { "correct": [ [ 2293 ], [ 1008 ], [ 105 ] ], "empty": [ [ 1573 ], [ 1008 ], [ 105 ] ], "reversed": [ [ 2293 ], [ 1008 ], [ 105 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BspMI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsrBI" ], "fragments": { "correct": [ 1801, 1364, 241 ], "empty": [ 1801, 644, 241 ], "reversed": [ 1801, 1364, 241 ] }, "bands": { "correct": [ [ 1801 ], [ 1364 ], [ 241 ] ], "empty": [ [ 1801 ], [ 644 ], [ 241 ] ], "reversed": [ [ 1801 ], [ 1364 ], [ 241 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsrDI" ], "fragments": { "correct": [ 3232, 174 ], "empty": [ 2512, 174 ], "reversed": [ 3232, 174 ] }, "bands": { "correct": [ [ 3232 ], [ 174 ] ], "empty": [ [ 2512 ], [ 174 ] ], "reversed": [ [ 3232 ], [ 174 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BsrFI" ], "fragments": { "correct": [ 1934, 1472 ], "empty": [ 2686 ], "reversed": [ 1886, 1520 ] }, "bands": { "correct": [ [ 1934 ], [ 1472 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 1886 ], [ 1520 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BssSI" ], "fragments": { "correct": [ 1384, 1104, 611, 307 ], "empty": [ 1384, 995, 307 ], "reversed": [ 1384, 965, 750, 307 ] }, "bands": { "correct": [ [ 1384 ], [ 1104 ], [ 611 ], [ 307 ] ], "empty": [ [ 1384 ], [ 995 ], [ 307 ] ], "reversed": [ [ 1384 ], [ 965 ], [ 750 ], [ 307 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "BstAPI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "BtsI" ], "fragments": { "correct": [ 1507, 1195, 684, 20 ], "empty": [ 1507, 1159, 20 ], "reversed": [ 1507, 1489, 390, 20 ] }, "bands": { "correct": [ [ 1507 ], [ 1195 ], [ 684 ] ], "empty": [ [ 1507 ], [ 1159 ] ], "reversed": [ [ 1507, 1489 ], [ 390 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "CviQI" ], "fragments": { "correct": [ 1773, 676, 434, 282, 241 ], "empty": [ 1769, 676, 241 ], "reversed": [ 2195, 676, 282, 241, 12 ] }, "bands": { "correct": [ [ 1773 ], [ 676 ], [ 434 ], [ 282 ], [ 241 ] ], "empty": [ [ 1769 ], [ 676 ], [ 241 ] ], "reversed": [ [ 2195 ], [ 676 ], [ 282 ], [ 241 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "DraI" ], "fragments": { "correct": [ 2695, 692, 19 ], "empty": [ 1975, 692, 19 ], "reversed": [ 2695, 692, 19 ] }, "bands": { "correct": [ [ 2695 ], [ 692 ] ], "empty": [ [ 1975 ], [ 692 ] ], "reversed": [ [ 2695 ], [ 692 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "DrdI" ], "fragments": { "correct": [ 1869, 1537 ], "empty": [ 1869, 817 ], "reversed": [ 1869, 1537 ] }, "bands": { "correct": [ [ 1869 ], [ 1537 ] ], "empty": [ [ 1869 ], [ 817 ] ], "reversed": [ [ 1869 ], [ 1537 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "EaeI" ], "fragments": { "correct": [ 1442, 987, 490, 389, 98 ], "empty": [ 1442, 987, 257 ], "reversed": [ 1442, 987, 389, 307, 281 ] }, "bands": { "correct": [ [ 1442 ], [ 987 ], [ 490 ], [ 389 ] ], "empty": [ [ 1442 ], [ 987 ], [ 257 ] ], "reversed": [ [ 1442 ], [ 987 ], [ 389 ], [ 307 ], [ 281 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "EarI" ], "fragments": { "correct": [ 1804, 1114, 488 ], "empty": [ 1804, 488, 394 ], "reversed": [ 1804, 1114, 488 ] }, "bands": { "correct": [ [ 1804 ], [ 1114 ], [ 488 ] ], "empty": [ [ 1804 ], [ 488 ], [ 394 ] ], "reversed": [ [ 1804 ], [ 1114 ], [ 488 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "EciI" ], "fragments": { "correct": [ 1507, 828, 568, 244, 146, 113 ], "empty": [ 1712, 828, 146 ], "reversed": [ 1374, 828, 701, 244, 146, 113 ] }, "bands": { "correct": [ [ 1507 ], [ 828 ], [ 568 ], [ 244 ], [ 146 ], [ 113 ] ], "empty": [ [ 1712 ], [ 828 ], [ 146 ] ], "reversed": [ [ 1374 ], [ 828 ], [ 701 ], [ 244 ], [ 146 ], [ 113 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": true, "empty-reversed": true }, "distinguishesAll": true }, { "enzymes": [ "Eco53kI" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, "correct-reversed": false, "empty-reversed": true }, "distinguishesAll": false }, { "enzymes": [ "EcoO109I" ], "fragments": { "correct": [ 3406 ], "empty": [ 2686 ], "reversed": [ 3406 ] }, "bands": { "correct": [ [ 3406 ] ], "empty": [ [ 2686 ] ], "reversed": [ [ 3406 ] ] }, "toldApart": { "correct-empty": true, 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