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"positive", "volume": 5e-05, "concentration": null, "carrierFraction": null }, { "well": "F3", "role": "positive", "source": "positive", "volume": 5e-05, "concentration": null, "carrierFraction": null }, { "well": "H12", "role": "blank", "source": "diluent", "volume": 5e-05, "concentration": 0.0, "carrierFraction": 0.0 } ], "totals": { "stock": 2.775e-06, "diluent": 0.001966225, "carrier": 3.5e-06, "deadVolume": 0.00018, "overage": 0.00015, "lastTubeSurplus": 9.25e-05, "leftover": 0.0004225, "doseWells": 24, "controlWells": 9 } } }, { "name": "refused-collision-dose", "about": "The CSO's adverse case: a vehicle well typed on a dose well collides; nothing is placed", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [ { "role": "vehicle", "wells": "A1, B3" } ], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "control-overlaps-dose", "detail": "B3" } }, { "name": "refused-collision-controls", "about": "Two controls on one well", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [ { "role": "vehicle", "wells": "E2:E7" }, { "role": "blank", "wells": "E7" } ], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "control-overlap", "detail": "E7" } }, { "name": "refused-token", "about": "A token that does not read is named", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [ { "role": "blank", "wells": "H12, Q1" } ], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "control-range", "detail": "Q1" } }, { "name": "refused-outside-plate", "about": "A well off a 96-well plate is named", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [ { "role": "blank", "wells": "I1:I3" } ], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "control-range", "detail": "I1:I3" } }, { "name": "refused-placement", "about": "Eight points along a row from B6 do not fit in 12 columns", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B6", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "placement" } }, { "name": "refused-start", "about": "A start well off the plate", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "J1", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "start" } }, { "name": "refused-stock-transfer-below-minimum", "about": "A 10 µM top from a 10 mM stock in 5 µL wells takes under 2 µL of stock: route it through an intermediate", "input": { "serial": { "top": { "value": 10, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 5, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "transfer-below-minimum", "detail": "tube-1" } }, { "name": "refused-intermediate-transfer-below-minimum", "about": "An intermediate whose own draw from the stock is under 2 µL", "input": { "serial": { "top": { "value": 20, "unit": "µM" }, "fold": "2", "points": 12, "replicates": 4, "wellVolume": { "value": 40, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": { "value": 1, "unit": "mM" }, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 384, "start": "C3", "direction": "columns", "controls": [], "deadVolume": { "value": 10, "unit": "µL" }, "overage": { "mode": "per-well", "percent": 5 } } }, "expected": { "expect": "refused", "problem": "transfer-below-minimum", "detail": "intermediate" } }, { "name": "refused-vehicle-carrier-below-minimum", "about": "Three vehicle wells need under 2 µL of neat DMSO", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [ { "role": "vehicle", "wells": "E2:E4" } ], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "transfer-below-minimum", "detail": "vehicle" } }, { "name": "refused-carrier-above-maximum", "about": "1% DMSO at the top against a 0.5% maximum", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": 0.5, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "carrier-above-maximum" } }, { "name": "refused-insufficient-stock", "about": "Less stock on hand than the sheet takes", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": { "value": 1, "unit": "µL" } }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "insufficient-stock" } }, { "name": "refused-intermediate-range", "about": "An intermediate weaker than the top", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": { "value": 50, "unit": "µM" }, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "intermediate" } }, { "name": "refused-stock-weaker", "about": "A stock weaker than the top", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 50, "unit": "µM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "stock-weaker-than-top" } }, { "name": "refused-overage", "about": "An overage above 1,000 percent", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": 20, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 1500 } } }, "expected": { "expect": "refused", "problem": "overage" } }, { "name": "refused-dead-volume", "about": "A negative dead volume", "input": { "serial": { "top": { "value": 100, "unit": "µM" }, "fold": "3", "points": 8, "replicates": 3, "wellVolume": { "value": 50, "unit": "µL" }, "stock": { "value": 10, "unit": "mM" }, "stockSolventFraction": "1", "solvent": "DMSO", "minimumTransfer": { "value": 2, "unit": "µL" }, "intermediate": null, "maxCarrierPercent": null, "stockAvailable": null }, "compound": "Compound 1", "sheet": { "v": 1, "format": 96, "start": "B2", "direction": "rows", "controls": [], "deadVolume": { "value": -1, "unit": "µL" }, "overage": { "mode": "bulk", "percent": 10 } } }, "expected": { "expect": "refused", "problem": "dead-volume" } } ] }