# Methods and checks

How each Drybench app reaches its results: which method every calculator, analysis and view uses, the references behind it, and how far each method has been checked.

[What the levels mean](https://www.drybench.com/methods/levels)

## Drybench Protein

Search a protein and see its experimental structure beside the AlphaFold model, and the drugs that act on it.

[Methods and checks for Drybench Protein](https://www.drybench.com/methods/protein) · [This page as Markdown](https://www.drybench.com/methods/protein/index.md)

- [Retained structure evidence](https://www.drybench.com/methods/protein#retained-structure-evidence): Tested, not reference-checked
- [Experimental structure evidence](https://www.drybench.com/methods/protein#experimental-structure-evidence): Tested, not reference-checked
- [Source confidence and exact residue transfer](https://www.drybench.com/methods/protein#source-confidence-and-exact-residue-transfer): Checked against a reference
- [Dossier](https://www.drybench.com/methods/protein#dossier): No method record is kept for this view, so it carries no level.
- [Compare](https://www.drybench.com/methods/protein#compare): No method record is kept for this view, so it carries no level.
- [Check a substitution](https://www.drybench.com/methods/protein#check-a-substitution): No method record is kept for this view, so it carries no level.
- [Compare with another entry](https://www.drybench.com/methods/protein#compare-with-another-entry): No method record is kept for this view, so it carries no level.

## Drybench Molecule

Draw or paste a molecule and see how drug-like it is, beside 2,197 approved drugs.

[Methods and checks for Drybench Molecule](https://www.drybench.com/methods/molecule) · [This page as Markdown](https://www.drybench.com/methods/molecule/index.md)

- [Scorecard](https://www.drybench.com/methods/molecule#scorecard): Tested, not reference-checked
- [Compare](https://www.drybench.com/methods/molecule#compare): Tested, not reference-checked

## Drybench Pipeline

Trials for any condition from ClinicalTrials.gov, US approvals from openFDA, and the FDA precedent behind them.

[Methods and checks for Drybench Pipeline](https://www.drybench.com/methods/pipeline) · [This page as Markdown](https://www.drybench.com/methods/pipeline/index.md)

- [Landscape](https://www.drybench.com/methods/pipeline#landscape): No method record is kept for this view, so it carries no level.
- [Trials](https://www.drybench.com/methods/pipeline#trials): No method record is kept for this view, so it carries no level.
- [Charts](https://www.drybench.com/methods/pipeline#charts): No method record is kept for this view, so it carries no level.
- [US approvals](https://www.drybench.com/methods/pipeline#us-approvals): No method record is kept for this view, so it carries no level.
- [FDA precedent](https://www.drybench.com/methods/pipeline#fda-precedent): Tested, not reference-checked

## Drybench Cell

From gene to working protein, in eight real human structures.

[Methods and checks for Drybench Cell](https://www.drybench.com/methods/cell) · [This page as Markdown](https://www.drybench.com/methods/cell/index.md)

- [The tour](https://www.drybench.com/methods/cell#the-tour): No method record is kept for this step, so it carries no level.

## Drybench Patent

Listed patents and exclusivities for a US product, each date traced to its Orange Book or Purple Book rule.

[Methods and checks for Drybench Patent](https://www.drybench.com/methods/patent) · [This page as Markdown](https://www.drybench.com/methods/patent/index.md)

- [Protection](https://www.drybench.com/methods/patent#protection): No method record is kept for this view, so it carries no level.
- [Patents](https://www.drybench.com/methods/patent#patents): No method record is kept for this view, so it carries no level.
- [Exclusivity](https://www.drybench.com/methods/patent#exclusivity): No method record is kept for this view, so it carries no level.
- [Generics and Paragraph IV](https://www.drybench.com/methods/patent#generics-and-paragraph-iv): No method record is kept for this view, so it carries no level.
- [Changes](https://www.drybench.com/methods/patent#changes): No method record is kept for this view, so it carries no level.
- [Date window](https://www.drybench.com/methods/patent#date-window): No method record is kept for this view, so it carries no level.

## Drybench Calc

Molarity, dilutions, serial plans, A280 and buffers with units on every field and the method beside each answer.

[Methods and checks for Drybench Calc](https://www.drybench.com/methods/calculators) · [This page as Markdown](https://www.drybench.com/methods/calculators/index.md)

- [Molarity and stocks](https://www.drybench.com/methods/calculators#molarity-and-stocks): Checked against a reference
- [Recipe table](https://www.drybench.com/methods/calculators#recipe-table): Checked against a reference
- [Percent solutions](https://www.drybench.com/methods/calculators#percent-solutions): Checked against a reference
- [Buffer pKa at temperature](https://www.drybench.com/methods/calculators#buffer-pka-at-temperature): Checked against a reference
- [C1V1 dilution](https://www.drybench.com/methods/calculators#c1v1-dilution): Checked against a reference
- [Serial dilution](https://www.drybench.com/methods/calculators#serial-dilution): Tested, not reference-checked
- [Antibody dilution](https://www.drybench.com/methods/calculators#antibody-dilution): Checked against a reference
- [Master mix](https://www.drybench.com/methods/calculators#master-mix): Checked against a reference
- [A280 and A260](https://www.drybench.com/methods/calculators#a280-and-a260): Checked against a reference
- [Cell seeding and splitting](https://www.drybench.com/methods/calculators#cell-seeding-and-splitting): Checked against a reference
- [Cell counting and viability](https://www.drybench.com/methods/calculators#cell-counting-and-viability): Checked against a reference
- [Cells from OD600 (estimate)](https://www.drybench.com/methods/calculators#cells-from-od600-estimate): Tested, not reference-checked
- [Dose per cell](https://www.drybench.com/methods/calculators#dose-per-cell): Checked against a reference
- [RCF and RPM](https://www.drybench.com/methods/calculators#rcf-and-rpm): Checked against a reference
- [Unit converter](https://www.drybench.com/methods/calculators#unit-converter): Checked against a reference

## Drybench Sequence

Reverse complement, translation, ORFs, protein MW and pI, and primer checks from one paste, with the method named.

[Methods and checks for Drybench Sequence](https://www.drybench.com/methods/sequence) · [This page as Markdown](https://www.drybench.com/methods/sequence/index.md)

- [Reverse complement](https://www.drybench.com/methods/sequence#reverse-complement): No method record is kept for this tool, so it carries no level.
- [Translate](https://www.drybench.com/methods/sequence#translate): Checked against a reference
- [Six frames](https://www.drybench.com/methods/sequence#six-frames): Checked against a reference
- [Open reading frames](https://www.drybench.com/methods/sequence#open-reading-frames): Checked against a reference
- [Protein MW and pI](https://www.drybench.com/methods/sequence#protein-mw-and-pi): Tested, not reference-checked
- [ε280](https://www.drybench.com/methods/sequence#ε280): Checked against a reference
- [GC percent](https://www.drybench.com/methods/sequence#gc-percent): No method record is kept for this tool, so it carries no level.
- [DNA molecular weight](https://www.drybench.com/methods/sequence#dna-molecular-weight): Checked against a reference
- [Motif search](https://www.drybench.com/methods/sequence#motif-search): No method record is kept for this tool, so it carries no level.
- [Oligo Tm](https://www.drybench.com/methods/sequence#oligo-tm): Checked against a reference
- [Primer check](https://www.drybench.com/methods/sequence#primer-check): Tested, not reference-checked
- [Primer design](https://www.drybench.com/methods/sequence#primer-design): Checked against a reference
- [Mutagenesis primers](https://www.drybench.com/methods/sequence#mutagenesis-primers): Checked against a reference
- [Review sequence revisions](https://www.drybench.com/methods/sequence#review-sequence-revisions): Checked against a reference
- [Find DNA fragment](https://www.drybench.com/methods/sequence#find-dna-fragment): Checked against a reference

## Drybench Assay

Dose-response, standard curves and qPCR with explicit methods and retained research context.

[Methods and checks for Drybench Assay](https://www.drybench.com/methods/assay) · [This page as Markdown](https://www.drybench.com/methods/assay/index.md)

- [Dose response](https://www.drybench.com/methods/assay#dose-response): Tested, not reference-checked
- [Weighted fit (1/Y²)](https://www.drybench.com/methods/assay#weighted-fit-1y2): Tested, not reference-checked
- [Plate quality](https://www.drybench.com/methods/assay#plate-quality): Tested, not reference-checked
- [Outlier candidates and influence](https://www.drybench.com/methods/assay#outlier-candidates-and-influence): Tested, not reference-checked
- [Curve shape and model choice](https://www.drybench.com/methods/assay#curve-shape-and-model-choice): Tested, not reference-checked
- [Comparing curves and experiments](https://www.drybench.com/methods/assay#comparing-curves-and-experiments): Tested, not reference-checked
- [Agonist and antagonist analyses](https://www.drybench.com/methods/assay#agonist-and-antagonist-analyses): Tested, not reference-checked
- [Reporter, viability and kinetic readings](https://www.drybench.com/methods/assay#reporter-viability-and-kinetic-readings): Tested, not reference-checked
- [Molar units and ligand efficiency](https://www.drybench.com/methods/assay#molar-units-and-ligand-efficiency): Tested, not reference-checked
- [Where to add a concentration](https://www.drybench.com/methods/assay#where-to-add-a-concentration): Tested, not reference-checked
- [Standard curve](https://www.drybench.com/methods/assay#standard-curve): Checked against a reference
- [qPCR relative expression](https://www.drybench.com/methods/assay#qpcr-relative-expression): Tested, not reference-checked
- [Growth: selected window](https://www.drybench.com/methods/assay#growth-selected-window): Tested, not reference-checked
- [Enzyme kinetics: initial rates](https://www.drybench.com/methods/assay#enzyme-kinetics-initial-rates): Tested, not reference-checked
- [Enzyme kinetics: inhibition](https://www.drybench.com/methods/assay#enzyme-kinetics-inhibition): Checked against a reference
- [Thermal shift: apparent Tm](https://www.drybench.com/methods/assay#thermal-shift-apparent-tm): Tested, not reference-checked
- [Screening campaign](https://www.drybench.com/methods/assay#screening-campaign): Tested, not reference-checked
- [Descriptive statistics](https://www.drybench.com/methods/assay#descriptive-statistics): Checked against a reference
- [Power and sample size](https://www.drybench.com/methods/assay#power-and-sample-size): Checked against a reference
- [Several groups: one factor](https://www.drybench.com/methods/assay#several-groups-one-factor): Checked against a reference
- [False discovery rate](https://www.drybench.com/methods/assay#false-discovery-rate): Checked against a reference
- [Survival curves](https://www.drybench.com/methods/assay#survival-curves): Checked against a reference
- [Compare proportions](https://www.drybench.com/methods/assay#compare-proportions): Checked against a reference
- [Correlation](https://www.drybench.com/methods/assay#correlation): Checked against a reference
- [GR metrics: GR50, GRmax and GR\_AOC](https://www.drybench.com/methods/assay#gr-metrics-gr50-grmax-and-graoc): Tested, not reference-checked
- [Competition and saturation binding](https://www.drybench.com/methods/assay#competition-and-saturation-binding): Checked against a reference
- [Combination responses](https://www.drybench.com/methods/assay#combination-responses): Checked against a reference

## Drybench Plasmid

What is in this plasmid, and which digest proves it: annotated maps, a schematic gel and sequencing-primer sites.

[Methods and checks for Drybench Plasmid](https://www.drybench.com/methods/plasmid) · [This page as Markdown](https://www.drybench.com/methods/plasmid/index.md)

- [Map](https://www.drybench.com/methods/plasmid#map): Tested, not reference-checked
- [Features](https://www.drybench.com/methods/plasmid#features): Tested, not reference-checked
- [Digest](https://www.drybench.com/methods/plasmid#digest): Tested, not reference-checked
- [Sequence](https://www.drybench.com/methods/plasmid#sequence): No method record is kept for this view, so it carries no level.
- [Numbers](https://www.drybench.com/methods/plasmid#numbers): Checked against a reference
- [Explainers](https://www.drybench.com/methods/plasmid#explainers): No method record is kept for this view, so it carries no level.
- [Assembly](https://www.drybench.com/methods/plasmid#assembly): Tested, not reference-checked
- [SpCas9 guide sites](https://www.drybench.com/methods/plasmid#spcas9-guide-sites): Checked against a reference

## Drybench Bioreactor

Batch, fed-batch and chemostat runs with cited presets, showing when oxygen runs out and what would rescue it.

[Methods and checks for Drybench Bioreactor](https://www.drybench.com/methods/bioreactor) · [This page as Markdown](https://www.drybench.com/methods/bioreactor/index.md)

- [Run](https://www.drybench.com/methods/bioreactor#run): Tested, not reference-checked
- [Rescue](https://www.drybench.com/methods/bioreactor#rescue): Tested, not reference-checked
- [Pump sheet](https://www.drybench.com/methods/bioreactor#pump-sheet): Checked against a reference
- [Measured data beside this run](https://www.drybench.com/methods/bioreactor#measured-data-beside-this-run): Checked against a reference
- [New comparison](https://www.drybench.com/methods/bioreactor#new-comparison): Checked against a reference
- [kLa calculator](https://www.drybench.com/methods/bioreactor#kla-calculator): Checked against a reference
- [OTR and OUR calculator](https://www.drybench.com/methods/bioreactor#otr-and-our-calculator): Checked against a reference
- [Scale-up calculator](https://www.drybench.com/methods/bioreactor#scale-up-calculator): Checked against a reference