Workflows · Version 1 · Example · pinned inputs
Stocks and dilution series
For a student or a new starter making their first stocks and plates.
Progress is kept on this device only.
What you will be able to do
- Track concentration, amount and volume through a serial transfer, and calculate the cumulative dilution.
- Calculate the mass to weigh for a molar stock of a salt form, correcting for purity, and state the parent-equivalent.
- Read tube, transfer and stock volumes for a serial plan from the fold, replicates and well volume, and see why each tube holds more than its wells take.
- Calculate the solvent carried into each point of a series, and decide when it needs a vehicle-matched series or a different stock.
The steps
1. Calc
Weigh a molar stock of the salt form, and know how purity changes the mass.
Before this step: Dilutions and Buffers
2. Calc
Plan a serial series and read what the solvent does down it.
3. Calc
Carry the series' logic to any transfer.
Before this step: Dilutions and Buffers
For a lecturer
A link for the class
Share this Workflow as a link. The settings travel in the link only; nothing is sent to a server.
Handouts
Print the steps and their questions for the class, or a copy with the answers for yourself.
Projector view
Larger text for a projected screen, in this tab only, until you turn it off or close the tab.